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Alkaloids derived from flowers of Senna spectabilis, (-)-cassine and (-)-spectaline, have antiproliferative activity on HepG2 cells for inducing cell cycle arrest in G1/S transition through ERK inactivation and downregulation of cyclin D1 expression.
Pereira, Rodrigo Machado; Ferreira-Silva, Guilherme Álvaro; Pivatto, Marcos; Santos, Luciana de Ávila; Bolzani, Vanderlan da Silva; Chagas de Paula, Daniela Aparecida; Oliveira, Jaqueline Carvalho de; Viegas Júnior, Cláudio; Ionta, Marisa.
Afiliação
  • Pereira RM; Institute of Biomedical Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, zip code 37130-000 Alfenas, MG, Brazil.
  • Ferreira-Silva GÁ; Institute of Biomedical Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, zip code 37130-000 Alfenas, MG, Brazil.
  • Pivatto M; Nucleus of Research in Natural Products (NuPPeN), Institute of Chemistry, Federal 'University of Uberlândia, Avenida João Naves de Ávila, 2121, zip code 38408-144 Uberlândia, MG, Brazil.
  • Santos Lde Á; Institute of Chemistry, State University of São Paulo, Rua Francisco Degni s/n, zip code 14801-970 Araraquara, SP, Brazil.
  • Bolzani Vda S; Institute of Chemistry, State University of São Paulo, Rua Francisco Degni s/n, zip code 14801-970 Araraquara, SP, Brazil.
  • Chagas de Paula DA; Laboratory of Phytochemistry and Medicinal Chemistry (LFQM), Institute of Chemistry, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, zip code 37130-000 Alfenas, MG, Brazil.
  • Oliveira JC; Institute of Natural Science, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, zip code 37130-000 Alfenas, MG, Brazil.
  • Viegas Júnior C; Laboratory of Research on Medicinal Chemistry (PeQuiM), Institute of Chemistry, Federal University of Alfenas, Av. Jovino Fernandes Sales, 2600, zip code 37130-000 Alfenas, MG, Brazil.
  • Ionta M; Institute of Biomedical Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, zip code 37130-000 Alfenas, MG, Brazil. Electronic address: marisaionta@gmail.com.
Toxicol In Vitro ; 31: 86-92, 2016 Mar.
Article em En | MEDLINE | ID: mdl-26616281
Cancer is one of the most critical problems of public health in the world and one of the main challenges for medicine in this century. Unfortunately, most patients are diagnosed at advanced stage, when the treatment options are palliative. Consequently, the search for novel therapeutic options is imperative. In the context, the plants represent an important source for discovering of novel compounds with pharmacological potential including antineoplastic agents. Herein, we aimed to investigate in vitro antiproliferative and cytotoxic potentials of an alkaloid mixture derived from Senna spectabilis, (−)-cassine (1) and (−)-spectaline (2). These alkaloids reduced cell viability in a concentration-dependent manner of six tumor cell lines. From initial screening, HepG2 cells were selected for further investigations. We show that alkaloids 1/2 have an important antiproliferative activity on HepG2 cells due to their ability in inducing cell cycle arrest in G1/S transition. This effect was associated to ERK inactivation and down-regulation of cyclin D1 expression. In addition, we evidenced a disruption of the microfilaments and microtubules in a consequence of the treatment. Taken together, the data showed by the first time that alkaloids 1/2 strongly inhibit cell proliferation of hepatocellular carcinoma cells. Therefore, they represent promise antitumor compounds against liver cancer and should be considered for further anticancer in vivo studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piperidinas / Cetonas / Antineoplásicos Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piperidinas / Cetonas / Antineoplásicos Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido