Your browser doesn't support javascript.
loading
Effect of SPL (Spent Pot Liner) and its main components on root growth, mitotic activity and phosphorylation of Histone H3 in Lactuca sativa L.
Freitas, Aline Silva; Fontes Cunha, Isabela Martinez; Andrade-Vieira, Larissa Fonseca; Techio, Vânia Helena.
Afiliação
  • Freitas AS; Department of Biology, Federal University of Lavras, P.O. Box 3037, 37.200-000 Lavras, Minas Gerais, Brasil.
  • Fontes Cunha IM; Department of Biology, Federal University of Lavras, P.O. Box 3037, 37.200-000 Lavras, Minas Gerais, Brasil.
  • Andrade-Vieira LF; Department of Biology, Federal University of Lavras, P.O. Box 3037, 37.200-000 Lavras, Minas Gerais, Brasil.
  • Techio VH; Department of Biology, Federal University of Lavras, P.O. Box 3037, 37.200-000 Lavras, Minas Gerais, Brasil. Electronic address: vhtechio@gmail.com.
Ecotoxicol Environ Saf ; 124: 426-434, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26615478
Spent Pot Liner (SPL) is a solid waste from the aluminum industry frequently disposed of in industrial landfills; it can be leached and contaminate the soil, sources of drinking water and plantations, and thus may pose a risk to human health and to ecosystems. Its composition is high variable, including cyanide, fluoride and aluminum salts, which are highly toxic and environmental pollutants. This study evaluated the effect of SPL and its main components on root growth and the mitosis of Lactuca sativa, by investigating the mechanisms of cellular and chromosomal alterations with the aid of immunolocalization. To this end, newly emerged roots of L. sativa were exposed to SPL and its main components (solutions of cyanide, fluoride and aluminum) and to calcium chloride (control) for 48h. After this, root length was measured and cell cycle was examined by means of conventional cytogenetics and immunolocalization. Root growth was inhibited in the treatments with SPL and aluminum; chromosomal and nuclear alterations were observed in all treatments. The immunolocalization evidenced normal dividing cells with regular temporal and spatial distribution of histone H3 phosphorylation at serine 10 (H3S10ph). However, SPL and its main components inhibited the phosphorylation of histone H3 at serine 10, inactivated pericentromeric regions and affected the cohesion of sister chromatids, thus affecting the arrangement of chromosomes in the metaphase plate and separation of chromatids in anaphase. In addition, these substances induced breaks in pericentromeric regions, characterized as fragile sites.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas / Raízes de Plantas / Resíduos Industriais / Mitose Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas / Raízes de Plantas / Resíduos Industriais / Mitose Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda