Novel analytical procedure using a combination of hollow fiber supported liquid membrane and dispersive liquid-liquid microextraction for the determination of aflatoxins in soybean juice by high performance liquid chromatography - Fluorescence detector.
Food Chem
; 196: 292-300, 2016 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-26593494
This study describes a combination between hollow fiber membrane and dispersive liquid-liquid microextraction for determination of aflatoxins in soybean juice by HPLC. The main advantage of this approach is the use of non-chlorinated solvent and small amounts of organic solvents. The optimum extraction conditions were 1-octanol as immobilized solvent; toluene and acetone at 1:5 ratio as extraction and disperser solvents (100 µL), NaCl at 2% of the sample volume and extraction time of 60 min. The optimal condition for the liquid desorption was 150 µL acetonitrile:water (50:50 v/v) and desorption time of 20 min. The linear range varied from 0.03 to 21 µg L(-1), with R(2) coefficients ranging from 0.9940 to 0.9995. The limits of detection and quantification ranged from 0.01 µg L(-1) to 0.03 µg L(-1) and from 0.03 µg L(-1) to 0.1 µg L(-1), respectively. Recovery tests ranged from 72% to 117% and accuracy between 12% and 18%.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glycine max
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Cromatografia Líquida de Alta Pressão
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Aflatoxinas
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Microextração em Fase Líquida
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido