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Electrochemical immunosensor for ethinylestradiol using diazonium salt grafting onto silver nanoparticles-silica-graphene oxide hybrids.
Cincotto, Fernando H; Martínez-García, Gonzalo; Yáñez-Sedeño, Paloma; Canevari, Thiago C; Machado, S A S; Pingarrón, José M.
Afiliação
  • Cincotto FH; Institute of Chemistry, State University of São Paulo, São Carlos, Brazil.
  • Martínez-García G; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, Madrid, Spain.
  • Yáñez-Sedeño P; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, Madrid, Spain.
  • Canevari TC; Engineering School, Mackenzie Presbiterian University, São Paulo, Brazil.
  • Machado SA; Institute of Chemistry, State University of São Paulo, São Carlos, Brazil.
  • Pingarrón JM; Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, Madrid, Spain.
Talanta ; 147: 328-34, 2016 Jan 15.
Article em En | MEDLINE | ID: mdl-26592615
This work describes the preparation of an electrochemical immunosensor for ethinylestradiol (EE2) based on grafting of diazonium salt of 4-aminobenzoic acid onto a glassy carbon electrode modified with silver nanoparticles/SiO2/graphene oxide hybrid followed by covalent binding of anti-ethinylestradiol (anti-EE2) to activated carboxyl groups. A competitive immunoassay was developed for the determination of the hormone using peroxidase-labeled ethinylestradiol (HRP-EE2) and measurement of the amperometric response at -200mV in the presence of hydroquinone (HQ) as redox mediator. The calibration curve for EE2 exhibited a linear range between 0.1 and 50ng/mL (r(2)=0.996), with a detection limit of 65pg/mL. Interference studies with other hormones related with EE2 revealed the practical specificity of the developed method for the analyte. A good reproducibility, with RSD=4.5% (n=10) was also observed. The operating stability of a single bioelectrode modified with anti-EE2 was maintained at least for 15 days when it was stored at 4°C under humid conditions between measurements. The developed immunosensor was applied to the analysis of spiked urine with good results.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prata / Imunoensaio / Técnicas Biossensoriais / Dióxido de Silício / Compostos de Diazônio / Etinilestradiol / Grafite Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prata / Imunoensaio / Técnicas Biossensoriais / Dióxido de Silício / Compostos de Diazônio / Etinilestradiol / Grafite Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda