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Does Leishmaniasis disease alter the parenchyma and protein expression in salivary glands?
Júnior, Aírton M C; de Amorim Carvalho, Fernando A; de Oliveira Dantas, Weslany; Gomes, Luana C L; da Silva, Andrezza B S; de Sousa Cavalcante, Maria M A; de Oliveira, Ingrid M; de Deus Moura de Lima, Marina; Rizzo, Márcia Dos Santos; de Carvalho Leite, Carla Maria; Moura, Selma Maria Dos Santos; de Deus Moura, Lúcia de Fátima Almeida; da Silva, Benedito B.
Afiliação
  • Júnior AM; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil airtoncondejr@uol.com.br.
  • de Amorim Carvalho FA; Department of Biochemistry and Pharmacology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • de Oliveira Dantas W; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • Gomes LC; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • da Silva AB; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • de Sousa Cavalcante MM; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • de Oliveira IM; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • de Deus Moura de Lima M; Department of Patology and Odontological Clinic, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • Rizzo Mdos S; Department of Clinical and Veterinary Surgery, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • de Carvalho Leite CM; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • Moura SM; Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • de Deus Moura Lde F; Department of Patology and Odontological Clinic, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
  • da Silva BB; Maternal Child Departament, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
Exp Biol Med (Maywood) ; 241(4): 359-66, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26568331
Leishmaniasis is considered a serious public health problem in several regions in Brazil and worldwide. This research aimed to perform a histopathological and proteomic study of parotid, submandibular and sublingual glands of BALB/c mice infected by Leishmania (L) infantum chagasi using histological, immunohistochemical and epifluorescence techniques. Twelve isogenic BALB/c male mice, around six- to eight-weeks old, were separated into two groups: the animals of the control group were injected with 0.15 ml of NaCl, while those in the experimental group were inoculated with 5 × 10(6) amastigote forms of Leishmania (L) infantum chagasi by the ip route. After 50 days, animals were euthanized and major salivary glands were collected to perform histological, immunohistochemical and epifluorescence techniques using anti-Caspase-2, anti-Ki-67 and anti-ß-catenin antibodies, respectively. The histological and morphometric evaluation showed clusters of mononuclear inflammatory cells and a higher area and perimeter of the parotid gland. However, none of the salivary glands had morphophysiological impairment. There was no immunoreactivity to the anti-caspase-2 antibody and Ki67 expression in acinar and ductal cells in both groups. According to the immunofluorescence staining, the ß-catenin antibodies did not show nuclear expression, suggesting no uncontrolled proliferation. The data obtained in this study showed population and morphological stability of major salivary glands after 50 days post-infection by Leishmania (L) infantum chagasi.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glândula Parótida / Glândula Sublingual / Glândula Submandibular / Leishmaniose Limite: Animals Idioma: En Revista: Exp Biol Med (Maywood) Assunto da revista: BIOLOGIA / FISIOLOGIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glândula Parótida / Glândula Sublingual / Glândula Submandibular / Leishmaniose Limite: Animals Idioma: En Revista: Exp Biol Med (Maywood) Assunto da revista: BIOLOGIA / FISIOLOGIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido