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Identification of molecular markers related to human alveolar bone cells and pathway analysis in diabetic patients.
Sun, X; Ren, Q H; Bai, L; Feng, Q.
Afiliação
  • Sun X; Department of Stomatology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan, China.
  • Ren QH; Department of Stomatology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan, China.
  • Bai L; Department of Stomatology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan, China.
  • Feng Q; Department of Stomatology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan, China.
Genet Mol Res ; 14(4): 13476-84, 2015 Oct 28.
Article em En | MEDLINE | ID: mdl-26535661
Alveolar bone osteoblasts are widely used in dental and related research. They are easily affected by systemic diseases such as diabetes. However, the mechanism of diabetes-induced alveolar bone absorption remains unclear. This study systematically explored the changes in human alveolar bone cell-related gene expression and biological pathways, which may facilitate the investigation of its mechanism. Alveolar bone osteoblasts isolated from 5 male diabetics and 5 male healthy adults were cultured. Total RNA was extracted from these cells and subjected to gene microarray analysis. Differentially expressed genes were screened, and a gene interaction network was constructed. An enrichment pathway analysis was simultaneously performed on differentially expressed genes to identify the biological pathways associated with changes in the alveolar bone cells of diabetic humans. In total, we identified 147 mRNAs that were differentially expressed in diabetic alveolar bone cells (than in the normal cells; 91 upregulated and 36 downregulated mRNAs). The constructed co-expression network showed 3 pairs of significantly-expressed genes. High-enrichment pathway analysis identified 8 pathways that were affected by changes in gene expression; three of the significant pathways were related to metabolism (inositol phosphate metabolism, propanoate metabolism, and pyruvate metabolism). Here, we identified a few potential genes and biological pathways for the diagnosis and treatment of alveolar bone cells in diabetic patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores / Diabetes Mellitus Tipo 2 / Processo Alveolar Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores / Diabetes Mellitus Tipo 2 / Processo Alveolar Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil