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Anti-steatotic effects of an n-3 LCPUFA and extra virgin olive oil mixture in the liver of mice subjected to high-fat diet.
Valenzuela, Rodrigo; Espinosa, Alejandra; Llanos, Paola; Hernandez-Rodas, Maria Catalina; Barrera, Cynthia; Vergara, Daniela; Romero, Nalda; Pérez, Francisco; Ruz, Manuel; Videla, Luis A.
Afiliação
  • Valenzuela R; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.
  • Espinosa A; Medical Technology Department, Faculty of Medicine, University of Chile, Santiago, Chile.
  • Llanos P; Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Santiago, Chile.
  • Hernandez-Rodas MC; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.
  • Barrera C; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.
  • Vergara D; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.
  • Romero N; Faculty of Chemical Sciences and Pharmacy, Department of Food Science and Chemical Technology, University of Chile, Santiago, Chile.
  • Pérez F; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.
  • Ruz M; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.
  • Videla LA; Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
Food Funct ; 7(1): 140-50, 2016 Jan.
Article em En | MEDLINE | ID: mdl-26471014
Non-alcoholic fatty liver disease (NAFLD) is characterized by liver steatosis, oxidative stress, and drastic depletion of n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), namely, eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n-3, DHA), which trigger lipolysis stimulation and lipogenesis inhibition. Extra virgin olive oil (EVOO) has important antioxidant effects. This study evaluated the anti-steatotic effects of n-3 LCPUFA plus EVOO in the liver of male C57BL/6J mice subjected to a control diet (CD) (10% fat, 20% protein, 70% carbohydrate) or high fat diet (HFD) (60% fat, 20% protein, 20% carbohydrate), without and with supplementation with n-3 LCPUFA (100 mg per kg per day) plus EVOO (100 mg per kg per day) for 12 weeks. HFD induced (i) liver steatosis (increased total fat, triacylglycerols, and free fatty acid total contents), (ii) higher fasting serum glucose and insulin levels and HOMA index, total cholesterol, triacylglycerols and TNF-α and IL-6, (iii) liver and plasma oxidative stress enhancement, (iv) depletion of the n-3 LCPUFA hepatic content, and (v) increment in lipogenic enzyme activity and reduction in lipolytic enzyme activity. These changes were either reduced (p < 0.05) or normalized to control the values in animals subjected to HFD supplemented with n-3 LCPUFA plus EVOO. In conclusion, n-3 LCPUFA plus EVOO intervention exerts anti-steatotic effects underlying antioxidant and anti-inflammatory responses, improved insulin sensitivity, and recovery of the lipolytic/lipogenic status of the liver altered by HFD, and supports the potential therapeutic use of n-3 LCPUFA plus EVOO supplementation in the treatment of human liver steatosis induced by nutritional factors or other etiologies.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Graxos Ômega-3 / Dieta Hiperlipídica / Hepatopatia Gordurosa não Alcoólica / Azeite de Oliva Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: Food Funct Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Graxos Ômega-3 / Dieta Hiperlipídica / Hepatopatia Gordurosa não Alcoólica / Azeite de Oliva Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: Food Funct Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido