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Resistance Risk Assessment of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Diatraea saccharalis (Lepidoptera: Crambidae) to Vip3Aa20 Insecticidal Protein Expressed in Corn.
Bernardi, Oderlei; Bernardi, Daniel; Amado, Douglas; Sousa, Renan S; Fatoretto, Julio; Medeiros, Fernanda C L; Conville, Jared; Burd, Tony; Omoto, Celso.
Afiliação
  • Bernardi O; Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP), Av., Pádua Dias 11, Piracicaba, São Paulo 13418-900, Brazil. oderleibernardi@yahoo.com.br.
  • Bernardi D; Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP), Av., Pádua Dias 11, Piracicaba, São Paulo 13418-900, Brazil.
  • Amado D; Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP), Av., Pádua Dias 11, Piracicaba, São Paulo 13418-900, Brazil.
  • Sousa RS; Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP), Av., Pádua Dias 11, Piracicaba, São Paulo 13418-900, Brazil.
  • Fatoretto J; Syngenta Crop Protection, Av. Nações Unidas 18001, São Paulo 04795-900, Brazil.
  • Medeiros FC; Syngenta Crop Protection, Av. Nações Unidas 18001, São Paulo 04795-900, Brazil.
  • Conville J; Syngenta Biotechnology Inc., Triangle Park P.O. Box 3054, Raleigh-Durham, NC 12257.
  • Burd T; Syngenta Crop Protection, Greenboro P.O. Box 18300, Greensboro, NC 27419.
  • Omoto C; Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP), Av., Pádua Dias 11, Piracicaba, São Paulo 13418-900, Brazil.
J Econ Entomol ; 108(6): 2711-9, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26470366
Transgenic Agrisure Viptera 3 corn that expresses Cry1Ab, Vip3Aa20, and EPSPS proteins and Agrisure Viptera expressing Vip3Aa20 are used for control of Spodoptera frugiperda (J.E. Smith) and Diatraea saccharalis (F.) in Brazil. To support a resistance management program, resistance risk assessment studies were conducted to characterize the dose expression of Vip3Aa20 protein and level of control against these species. The Vip3Aa20 expression in Agrisure Viptera 3 and Agrisure Viptera decreased from V6 to V10 stage of growth. However, Vip3Aa20 expression in Agrisure Viptera 3 at V6 and V10 stages was 13- and 16-fold greater than Cry1Ab, respectively. The Vip3Aa20 expression in lyophilized tissue of Agrisure Viptera 3 and Agrisure Viptera diluted 25-fold in an artificial diet caused complete larval mortality of S. frugiperda and D. saccharalis. In contrast, lyophilized tissue of Bt11 at the same dilution does not provide complete mortality of these species. Agrisure Viptera 3 and Agrisure Viptera also caused a high level of mortality against S. frugiperda and D. saccharalis. Moreover, 100% mortality was observed for S. frugiperda larvae (neonates through fifth-instar larvae) when fed in corn with the Vip trait technology. Viptera corn achieves a high level of control against S. frugiperda and D. saccharalis providing a high dose, which is an important determination to support the refuge strategy for an effective resistance management program.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Spodoptera Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Econ Entomol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Spodoptera Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Econ Entomol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido