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Optimization and validation method to evaluate the residues of ß-lactams and tetracyclines in kidney tissue by UPLC-MS/MS.
de Almeida, Marcos Pego; Rezende, Cristiana Perdigão; Ferreira, Flavia Domingues; de Souza, Leonardo Francisco; de Assis, Débora Cristina Sampaio; de Figueiredo, Tadeu Chaves; de Oliveira Leite, Mônica; de Vasconcelos Cançado, Silvana.
Afiliação
  • de Almeida MP; Laboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano, s/n, Pedro Leopoldo, MG CEP 33600-000, Brazil.
  • Rezende CP; Laboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano, s/n, Pedro Leopoldo, MG CEP 33600-000, Brazil.
  • Ferreira FD; Laboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano, s/n, Pedro Leopoldo, MG CEP 33600-000, Brazil.
  • de Souza LF; Laboratório Nacional Agropecuário (Lanagro-MG), Av. Rômulo Joviano, s/n, Pedro Leopoldo, MG CEP 33600-000, Brazil.
  • de Assis DC; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, MG CEP 30123-970, Brazil.
  • de Figueiredo TC; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, MG CEP 30123-970, Brazil.
  • de Oliveira Leite M; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, MG CEP 30123-970, Brazil.
  • de Vasconcelos Cançado S; Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, MG CEP 30123-970, Brazil. Electronic address: silvanavc@ufmg.br.
Talanta ; 144: 922-32, 2015 Nov 01.
Article em En | MEDLINE | ID: mdl-26452909
Methods are validated by a process that defines the analytical requirements and confirms that the investigated method is capable of performing consistently. A quantitative and confirmatory method for determining the presence of ß-lactam and tetracycline multiresidues in avian, bovine, equine, and swine kidney tissues using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed, optimized, and validated. Analytes were extracted from the kidneys by a mixture of water and acetonitrile, and the extract was then purified with hexane and C18 (dispersive phase). The method was evaluated by the following parameters: linearity, matrix effect, specificity, decision limits (CCα), detection capability (CCß), accuracy, precision, trueness, limits of detection (LOD), limits of quantification (LOQ), and robustness. The validated method presented a broad linear study range and significant matrix effect. The limit of detection (LOD) was defined from 2.5 to 25.0 µg kg(-1), and the limit of quantification (LOQ) was defined from 5.0 to 50.0 µg kg(-1) for individual analytes. The resultant recovery values ranged from 98.1% to 107.3% in repeatability conditions and from 95.2% to 106% under intralaboratory reproducibility conditions for the studied analytes. It was concluded that the performance parameters demonstrated total method adequacy for detecting and quantifying ß-lactam and tetracycline residues in swine, equine, bovine, and avian kidneys.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tetraciclinas / Beta-Lactamas / Rim / Antibacterianos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Talanta Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tetraciclinas / Beta-Lactamas / Rim / Antibacterianos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Talanta Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda