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Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats.
Han, Xufeng; Wang, Lei; Li, Wei; Li, Bibo; Yang, Yuxin; Yan, Hailong; Qu, Lei; Chen, Yulin.
Afiliação
  • Han X; College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
  • Wang L; College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
  • Li W; College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
  • Li B; College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
  • Yang Y; College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
  • Yan H; College of Life Science, Yulin University, People's Republic of China, China .
  • Qu L; College of Life Science, Yulin University, People's Republic of China, China .
  • Chen Y; College of Animal Science and Technology, Northwest A&F University, People's Republic of China, China .
Braz J Microbiol ; 46(3): 849-54, 2015.
Article em En | MEDLINE | ID: mdl-26413069
The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rúmen / Cabras / Lactobacillus plantarum / Duodeno Limite: Animals Idioma: En Revista: Braz J Microbiol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rúmen / Cabras / Lactobacillus plantarum / Duodeno Limite: Animals Idioma: En Revista: Braz J Microbiol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil