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Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.
Han, X G; Wang, D K; Gao, F; Liu, R H; Bi, Z G.
Afiliação
  • Han XG; Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
  • Wang DK; Department of Radiology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
  • Gao F; The Veins Allocation Center of Clinic Service, First Affiliated Hospital of Harbin Medical University, Harbin.
  • Liu RH; Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
Genet Mol Res ; 14(3): 11063-72, 2015 Sep 21.
Article em En | MEDLINE | ID: mdl-26400336
Bone morphogenetic protein 2 (BMP-2) can promote fracture healing. Although the complex role BMP-2 in bone formation is increasingly understood, the role of endogenous BMP-2 in nonunion remains unclear. Decorin (DCN) can promote the formation of bone matrix and calcium deposition to control bone morphogenesis. In this study, tissue composition and expression of BMP-2 and DCN were detected in different parts of old fracture zones to explore inherent anti-fibrotic ability and osteogenesis. Twenty-three patients were selected, including eight cases of delayed union and 15 cases of nonunion. Average duration of delayed union or nonunion was 15 months. Fracture fragments and surrounding tissues, including bone grafts, marrow cavity contents, and sticking scars, were categorically sampled during surgery. Through observation and histological testing, component comparisons were made between fracture fragments and surrounding tissue. The expression levels of DCN and BMP-2 in different tissues were detected by immunohistochemical staining and real-time polymerase chain reaction. The expression of DCN and BMP- 2 in different parts of the nonunion area showed that, compared with bone graft and marrow cavity contents, sticking scars had the highest expression of BMP-2. Compared with the marrow cavity contents and sticking scars, bone grafts had the highest expression of DCN. The low antifibrotic and osteogenic activity of the nonunion area was associated with non-co-expression of BMP-2 and DCN. Therefore, the co-injection of osteogenic factor BMP and DCN into the nonunion area can improve the induction of bone formation and enhance the conversion of the old scar, thereby achieving better nonunion treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osso e Ossos / Proteína Morfogenética Óssea 2 / Decorina / Fraturas não Consolidadas Limite: Adult / Aged / Aged80 / Humans / Middle aged Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osso e Ossos / Proteína Morfogenética Óssea 2 / Decorina / Fraturas não Consolidadas Limite: Adult / Aged / Aged80 / Humans / Middle aged Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil