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Identification of target genes for adenohypophysis-prefer miR-7 and miR-375 in cattle.
Yuan, B; Sun, G J; Zhang, G L; Wu, J; Xu, C; Dai, L S; Chen, J; Yu, X F; Zhao, Z H; Zhang, J B.
Afiliação
  • Yuan B; College of Animal Sciences, Jilin University, Changchun, China.
  • Sun GJ; College of Animal Sciences, Jilin University, Changchun, China.
  • Zhang GL; Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, China.
  • Wu J; Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, China.
  • Xu C; College of Animal Sciences, Jilin University, Changchun, China.
  • Dai LS; College of Animal Sciences, Jilin University, Changchun, China.
  • Chen J; College of Animal Sciences, Jilin University, Changchun, China.
  • Yu XF; College of Animal Sciences, Jilin University, Changchun, China.
  • Zhao ZH; College of Animal Sciences, Jilin University, Changchun, China.
  • Zhang JB; College of Animal Sciences, Jilin University, Changchun, China.
Genet Mol Res ; 14(3): 9753-63, 2015 Aug 19.
Article em En | MEDLINE | ID: mdl-26345908
In this study, expression levels of miRNAs (miRNAs), miR-375 and miR-7, were detected in different tissues of cattle to determine whether adenohypophysis-prefer or exclusively expressed miRNAs, and target genes could be predicted by TargetScan, RNA22, and other software. Target genes related to pituitary function or reproductive traits were identified using a dual-luciferase assay. miR-375 and miR-7 were expressed differently in various tissues. miR-375 and miR-7 showed higher expression in the adenohypophysis, and there was a significant difference compared with expression in other tissues (P < 0.01). The binding sites for miR-7 were the mRNAs of bone morphogenetic protein receptor type II (BMPR2), prostaglandin F2 receptor negative regulator, gonadotropin-releasing hormone receptor, follicle-stimulating hormoneß, somatostatin receptor 1, and interleukin-1ß by bioinformatic analysis; similarly, the mRNAs of BMPR2 and leptin contained binding sites for miR-375, suggesting that these genes are affected by miR-7 or miR-375. Dual-luciferase reporter assays showed that miR-7 regulated prostaglandin F2 receptor negative regulator expression, while miR-375 regulated BMPR2 expression. The mutated plasmid and miRNA mimics were used to co-transfect NIH3T3 cells; luciferase reporter assays showed that the inhibition of luciferase activity in the wild-type cells dramatically decreased from 75 to 26% with a 3-5-nucleotide mismatch mutation into the seed region of miR-7. miR-375 had nearly lost the ability to inhibit luciferase activity, suggesting that GTCTTCC is the site of interaction between miR-7 and the prostaglandin F2 receptor negative regulator sequence and that GAACAAA is the site of interaction between miR-375 and the BMPR2 sequence.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adeno-Hipófise / RNA Mensageiro / MicroRNAs Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adeno-Hipófise / RNA Mensageiro / MicroRNAs Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil