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Internalization of titanium dioxide nanoparticles by glial cells is given at short times and is mainly mediated by actin reorganization-dependent endocytosis.
Huerta-García, Elizabeth; Márquez-Ramírez, Sandra Gissela; Ramos-Godinez, María Del Pilar; López-Saavedra, Alejandro; Herrera, Luis Alonso; Parra, Alberto; Alfaro-Moreno, Ernesto; Gómez, Erika Olivia; López-Marure, Rebeca.
Afiliação
  • Huerta-García E; Departamento de Fisiología (Biología Celular), Instituto Nacional de Cardiología "Ignacio Chávez", Mexico; Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.
  • Márquez-Ramírez SG; Departamento de Fisiología (Biología Celular), Instituto Nacional de Cardiología "Ignacio Chávez", Mexico; Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.
  • Ramos-Godinez Mdel P; Departamento de Microscopía Electrónica, Instituto Nacional de Cancerología, Mexico.
  • López-Saavedra A; Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología, Mexico.
  • Herrera LA; Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología, Mexico; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico.
  • Parra A; Departamento de Inmunología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.
  • Alfaro-Moreno E; Laboratorio de Toxicología Ambiental, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Mexico(h); Swedish Toxicology Sciences Research Center (Swetox), Södertälje, Sweden.
  • Gómez EO; Academia de Biología, Colegio de Ciencias y Humanidades, Universidad Autónoma de la Ciudad de México, Mexico.
  • López-Marure R; Departamento de Fisiología (Biología Celular), Instituto Nacional de Cardiología "Ignacio Chávez", Mexico. Electronic address: rlmarure@yahoo.com.mx.
Neurotoxicology ; 51: 27-37, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26340880
Many nanoparticles (NPs) have toxic effects on multiple cell lines. This toxicity is assumed to be related to their accumulation within cells. However, the process of internalization of NPs has not yet been fully characterized. In this study, the cellular uptake, accumulation, and localization of titanium dioxide nanoparticles (TiO2 NPs) in rat (C6) and human (U373) glial cells were analyzed using time-lapse microscopy (TLM) and transmission electron microscopy (TEM). Cytochalasin D (Cyt-D) was used to evaluate whether the internalization process depends of actin reorganization. To determine whether the NP uptake is mediated by phagocytosis or macropinocytosis, nitroblue tetrazolium (NBT) reduction was measured and the 5-(N-ethyl-N-isopropyl)-amiloride was used. Expression of proteins involved with endocytosis and exocytosis such as caveolin-1 (Cav-1) and cysteine string proteins (CSPs) was also determined using flow cytometry. TiO2 NPs were taken up by both cell types, were bound to cellular membranes and were internalized at very short times after exposure (C6, 30 min; U373, 2h). During the uptake process, the formation of pseudopodia and intracellular vesicles was observed, indicating that this process was mediated by endocytosis. No specific localization of TiO2 NPs into particular organelles was found: in contrast, they were primarily localized into large vesicles in the cytoplasm. Internalization of TiO2 NPs was strongly inhibited by Cyt-D in both cells and by amiloride in U373 cells; besides, the observed endocytosis was not associated with NBT reduction in either cell type, indicating that macropinocytosis is the main process of internalization in U373 cells. In addition, increases in the expression of Cav-1 protein and CSPs were observed. In conclusion, glial cells are able to internalize TiO2 NPs by a constitutive endocytic mechanism which may be associated with their strong cytotoxic effect in these cells; therefore, TiO2 NPs internalization and their accumulation in brain cells could be dangerous to human health.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Titânio / Neuroglia / Actinas / Endocitose / Nanopartículas Metálicas Limite: Animals / Humans Idioma: En Revista: Neurotoxicology Ano de publicação: 2015 Tipo de documento: Article País de afiliação: México País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Titânio / Neuroglia / Actinas / Endocitose / Nanopartículas Metálicas Limite: Animals / Humans Idioma: En Revista: Neurotoxicology Ano de publicação: 2015 Tipo de documento: Article País de afiliação: México País de publicação: Holanda