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Novel genetic factors involved in resistance to Bacillus thuringiensis in Plutella xylostella.
Ayra-Pardo, C; Raymond, B; Gulzar, A; Rodríguez-Cabrera, L; Morán-Bertot, I; Crickmore, N; Wright, D J.
Afiliação
  • Ayra-Pardo C; Environmental Biotechnology Group, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
  • Raymond B; Division of Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire, UK.
  • Gulzar A; Division of Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire, UK.
  • Rodríguez-Cabrera L; Environmental Biotechnology Group, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
  • Morán-Bertot I; Environmental Biotechnology Group, Centre for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
  • Crickmore N; School of Life Sciences, University of Sussex, Brighton, UK.
  • Wright DJ; Division of Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, Silwood Park Campus, Ascot, Berkshire, UK.
Insect Mol Biol ; 24(6): 589-600, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26335439
The widespread and sustainable exploitation of the entomopathogen Bacillus thuringiensis (Bt) in pest control is threatened by the evolution of resistance. Although resistance is often associated with loss of binding of the Bt toxins to the insect midgut cells, other factors have been implicated. Here we used suppressive subtractive hybridization and gene expression suppression to identify additional molecular components involved in Bt-resistance in Plutella xylostella. We isolated transcripts from genes that were differentially expressed in the midgut of larvae from a resistant population, following ingestion of a Bt kurstaki HD1 strain-based commercial formulation (DiPel), and compared with a genetically similar susceptible population. Quantitative real-time polymerase-chain reaction (RT-PCR) analysis confirmed the differential basal expression of a subset of these genes. Gene expression suppression of three of these genes (P. xylostella cyclin-dependent kinase 5 regulatory subunit associated protein 1-like 1, stromal cell-derived factor 2-like 1 and hatching enzyme-like 1) significantly increased the pathogenicity of HD1 to the resistant population. In an attempt to link the multitude of factors reportedly influencing resistance to Bt with the well-characterized loss of toxin binding, we also considered Bt-resistance models in P. xylostella and other insects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Resistência a Inseticidas / Proteínas de Insetos / Agentes de Controle Biológico / Mariposas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Insect Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Cuba País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Resistência a Inseticidas / Proteínas de Insetos / Agentes de Controle Biológico / Mariposas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Insect Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Cuba País de publicação: Reino Unido