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Inhibitory effect of GB-2a (I3-naringenin-II8-eriodictyol) on melanogenesis.
Campos, Patrícia Mazureki; Prudente, Arthur Silveira; Horinouchi, Cíntia Delai da Silva; Cechinel-Filho, Valdir; Fávero, Giovani Marino; Cabrini, Daniela Almeida; Otuki, Michel Fleith.
Afiliação
  • Campos PM; Department of Pharmaceutical Sciences, Federal University of Parana, CEP 80210-170, Curitiba, PR, Brazil.
  • Prudente AS; Department of Pharmacology, Federal University of Parana, CEP 81530-900, Curitiba, PR, Brazil.
  • Horinouchi CD; Department of Pharmacology, Federal University of Parana, CEP 81530-900, Curitiba, PR, Brazil.
  • Cechinel-Filho V; Investigation Centre of Pharmaceutical Chemistry, University of Itajai Valley, CEP 88302-202, Itajai, SC, Brazil.
  • Fávero GM; Department of Biological Sciences, State University of Ponta Grossa, CEP 84030-900, Ponta Grossa, PR, Brazil.
  • Cabrini DA; Department of Pharmacology, Federal University of Parana, CEP 81530-900, Curitiba, PR, Brazil.
  • Otuki MF; Department of Pharmacology, Federal University of Parana, CEP 81530-900, Curitiba, PR, Brazil. Electronic address: michelotuki@yahoo.com.br.
J Ethnopharmacol ; 174: 224-9, 2015 Nov 04.
Article em En | MEDLINE | ID: mdl-26297636
ETHNOPHARMACOLOGY RELEVANCE: GB-2a is a I3-naringenin-II8-eriodictyol compound isolated from Garcinia gardneriana (Planchon & Triana) Zappi, a plant used in folk medicine for the treatment of skin disorders. AIM OF STUDY: In the search for new depigmenting agents, this study was carried out to investigate the in vitro effects of GB-2a isolated from G. gardneriana (Planchon & Triana) Zappi in B16F10 melanoma cells. MATERIALS AND METHODS: The effects of GB-2a were evaluated through determination of melanin biosynthesis in B16F10 melanoma cells in comparison with the reference drug kojic acid (500µM). In parallel, the GB-2a effect was assessed in a cell viability assay. Mushroom tyrosinase activity assays were conducted to verify the effect of this enzyme. In order to ascertain the nature of enzyme inhibition on tyrosinase, kinetics analysis of the GB-2a was performed with L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) substrates. RESULTS: The results showed that GB-2a biflavonoid significantly inhibited the melanin content, without reducing cell viability. GB-2a also showed a strong antityrosinase activity in the mushroom tyrosinase assay. GB-2a inhibited the tyrosinase activity, exerting a mixed inhibition. For the L-tyrosine substrate the inhibition was in non-competitive mode and for L-DOPA it was in uncompetitive mode. CONCLUSION: GB-2a biflavonoid promoted inhibition on tyrosinase activity and reduced melanin biosynthesis in B16F10 cells, which suggests great potential for medical and cosmetic uses as a depigmenting agent.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Melanoma Experimental / Extratos Vegetais / Folhas de Planta / Garcinia / Flavanonas / Melaninas Limite: Animals Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Melanoma Experimental / Extratos Vegetais / Folhas de Planta / Garcinia / Flavanonas / Melaninas Limite: Animals Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda