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Identification and genetic characterization of Piscirickettsia salmonis in native fish from southern Chile.
Contreras-Lynch, Sergio; Olmos, Paola; Vargas, Augusto; Figueroa, Jaime; González-Stegmaier, Roxana; Enríquez, Ricardo; Romero, Alex.
Afiliação
  • Contreras-Lynch S; Instituto de Fomento Pesquero, Puerto Montt 5480000, Chile.
Dis Aquat Organ ; 115(3): 233-44, 2015 Aug 20.
Article em En | MEDLINE | ID: mdl-26290508
Piscirickettsia salmonis is the etiological agent of piscirickettsiosis, a severe disease causing high mortalities in salmonids. This bacterium has been previously identified and isolated in all cultivated salmonids in Chile and worldwide, including Salmo salar, Oncorhynchus kisutch, and O. mykiss, in addition to being found in non-salmonid species such as Dicentrarchus labrax and Atractoscion nobilis. In this study, the 16S rRNA gene and intergenic spacer ITS-1 of P. salmonis were amplified by PCR from DNA samples extracted from the native Chilean fish species Eleginops maclovinus, Odontesthes regia, Sebastes capensis, and Salilota australis. Analysis of the 16S rRNA sequences from O. regia demonstrated a close phylogenetic relationship with the 16S rRNA gene in the Chilean EM-90 strain. The 16S rRNA sequences from E. maclovinus, S. capensis, and S. australis were related to the Chilean LF-89 sequence and Scottish strains. To confirm these findings, analysis of P. salmonis ITS-1 sequences obtained from the 4 sampled native species demonstrated a high degree of identity and a close phylogenetic relationship with Chilean P. salmonis sequences, including LF-89 and EM-90. These results suggest a strong relationship between the nucleotide sequences from the 16S rRNA and ITS-1 genes amplified from native fish with those sequences described in the first P. salmonis strains to be identified and isolated in Chile.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Piscirickettsiaceae / Piscirickettsia / Doenças dos Peixes Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: Dis Aquat Organ Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Chile País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Piscirickettsiaceae / Piscirickettsia / Doenças dos Peixes Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: Dis Aquat Organ Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Chile País de publicação: Alemanha