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Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder.
Chantre-Justino, Mariana; Alves, Gilda; Britto, Constança; Cardoso, Angélica; Scherrer, Luciano; Moreira, Aline dos Santos; Quirino, Raul; Ornellas, Antonio; Leitão, Alvaro; Lage, Claudia.
Afiliação
  • Chantre-Justino M; Carlos Chagas Filho Institute of Biophysics, Rio de Janeiro Federal University, Rio de Janeiro, Brazil.
  • Alves G; Research Coordination, National Institute of Cancer (INCA), Rio de Janeiro, Brazil.
  • Britto C; Molecular Biology and Endemic Diseases Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.
  • Cardoso A; Molecular Biology and Endemic Diseases Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.
  • Scherrer L; Brazilian Society of Clinical Oncology, Belo Horizonte, Minas Gerais, Brazil.
  • Moreira Ados S; DNA Sequencing Platform, PDTIS/FIOCRUZ, Rio de Janeiro, Brazil.
  • Quirino R; Department of Urology, National Institute of Cancer, Rio de Janeiro, Brazil.
  • Ornellas A; Department of Urology, National Institute of Cancer, Rio de Janeiro, Brazil.
  • Leitão A; Carlos Chagas Filho Institute of Biophysics, Rio de Janeiro Federal University, Rio de Janeiro, Brazil.
  • Lage C; Carlos Chagas Filho Institute of Biophysics, Rio de Janeiro Federal University, Rio de Janeiro, Brazil.
Oncol Rep ; 34(4): 1667-74, 2015 Oct.
Article em En | MEDLINE | ID: mdl-26238022
Molecular evidence indicates that alterations in genes involved in the maintenance of genome stability may be related to susceptibility to bladder carcinoma. Our goal was to evaluate the prognostic role of base excision repair (BER) genes in a cohort of patients diagnosed with primary urothelial carcinoma of the bladder (UCB). The levels of all APE1, XRCC1 and POLB transcripts were detected by quantitative real-time PCR (qPCR) technique in tumor samples from 52 patients undergoing transurethral resection (TUR) for primary UCB at the Department of Urology, Brazilian National Cancer Institute, Rio de Janeiro. Increased levels of APE1, XRCC1 and POLB transcripts were significantly associated with high-grade tumors when compared to these levels in low-grade tumors (p<0.01) and could be attributed to different mechanisms of transcriptional regulation as a response to tumorigenesis and oxidative stress. By analyzing the collected data in the present study, regardless of pathological grade or stage, univariate analysis revealed that the reduced levels of APE1 transcripts were significantly associated with cancer-specific mortality (p=0.032). Furthermore, the variant genotype (TG/GG) of the APE1 T1349G polymorphism was observed in 75% of a subset of patients who concomitantly experienced reduced levels of the APE1 transcript and death and/or recurrence events. Taken together, our data reinforce the idea that human DNA repair mechanisms must be finely regulated in order to avoid instability leading to tumorigenesis and poor clinical outcomes in UCB patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Bexiga Urinária / DNA Polimerase beta / DNA Liase (Sítios Apurínicos ou Apirimidínicos) / Proteínas de Ligação a DNA / Recidiva Local de Neoplasia Tipo de estudo: Prognostic_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Oncol Rep Assunto da revista: NEOPLASIAS Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Grécia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Bexiga Urinária / DNA Polimerase beta / DNA Liase (Sítios Apurínicos ou Apirimidínicos) / Proteínas de Ligação a DNA / Recidiva Local de Neoplasia Tipo de estudo: Prognostic_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Oncol Rep Assunto da revista: NEOPLASIAS Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Grécia