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Cytokine measurements in gingival crevicular fluid and periodontal ligament: Are they correlated?
Madureira, Davidson Fróis; da Silva, Janine Mayra; Teixeira, Antônio Lúcio; Abreu, Mauro Henrique Nogueira Guimarães; Pretti, Henrique; Lages, Elizabeth Maria Bastos; da Silva, Tarcília Aparecida.
Afiliação
  • Madureira DF; Postgraduate student, Department of Morphology, Institute of Biologic Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • da Silva JM; Postgraduate student, Department of Morphology, Institute of Biologic Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Teixeira AL; Associate professor, Department of Biochemistry and Immunology, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Abreu MH; Associate professor, Department of Community and Preventive Dentistry, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Pretti H; Associate professor, Department of Pediatric Dentistry and Orthodontics, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Lages EM; Associate professor, Department of Pediatric Dentistry and Orthodontics, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • da Silva TA; Associate professor, Department of Oral Surgery and Pathology, Faculty of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: tarcilia@ufmg.br.
Am J Orthod Dentofacial Orthop ; 148(2): 293-301, 2015 Aug.
Article em En | MEDLINE | ID: mdl-26232838
INTRODUCTION: Mechanical stress can induce molecular changes in gingival crevicular fluid (GCF) and the periodontal ligament (PDL). It is still not clear whether changes in the PDL and GCF are linked. In this study, we aimed to analyze the expression of cytokines in GCF and PDL after mechanical stress. METHODS: Twenty-three healthy patients were included. The experimental group consisted of premolars subjected to a force of 0.980 N for 1, 3, 7, 14, 21, or 28 days. The contralateral teeth were the controls. GCF and PDL samples were collected at the same time points for analysis of cytokines using the cytometric bead array. RESULTS: Interleukin (IL)-6 (IL-6) production was significantly elevated in the PDL on day 1 after force application. Significantly strong positive correlations between GCF and PDL in experimental group were seen on days 3 (interferon-gamma), 7 (IL-10), 14 (IL-17A), and 28 (IL-17A, tumor necrosis factor-alpha), and significantly strong negative correlation were seen on days 14 (interferon-gamma) and 21 (IL-2, IL-10). CONCLUSIONS: Different patterns of IL-6 expression were seen in the PDL and GCF after mechanical stress. Despite occasional correlations between GCF and PDL, the molecular contributions of the PDL to the GCF changes could not be clearly defined by our model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ligamento Periodontal / Citocinas / Líquido do Sulco Gengival Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Child / Female / Humans / Male Idioma: En Revista: Am J Orthod Dentofacial Orthop Assunto da revista: ODONTOLOGIA / ORTODONTIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ligamento Periodontal / Citocinas / Líquido do Sulco Gengival Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Child / Female / Humans / Male Idioma: En Revista: Am J Orthod Dentofacial Orthop Assunto da revista: ODONTOLOGIA / ORTODONTIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos