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Gene expression profile of ABC transporters and cytotoxic effect of ibuprofen and acetaminophen in an epithelial ovarian cancer cell line in vitro.
Lima, Renilton Aires; Cândido, Eduardo Batista; de Melo, Flávia Perrim; Piedade, Josiane Barbosa; Vidigal, Paula Vieira Teixeira; Silva, Luciana Maria; da Silva Filho, Agnaldo Lopes.
Afiliação
  • Lima RA; Hospital das Clínicas de Minas Gerais, Belo Horizonte, MG, Brazil.
  • Cândido EB; Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
  • de Melo FP; Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Piedade JB; Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Vidigal PV; Department of Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
  • Silva LM; Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • da Silva Filho AL; Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Rev Bras Ginecol Obstet ; 37(6): 283-90, 2015 Jun.
Article em En | MEDLINE | ID: mdl-26200827
PURPOSES: To determine the basic expression of ABC transporters in an epithelial ovarian cancer cell line, and to investigate whether low concentrations of acetaminophen and ibuprofen inhibited the growth of this cell line in vitro. METHODS: TOV-21 G cells were exposed to different concentrations of acetaminophen (1.5 to 15 µg/mL) and ibuprofen (2.0 to 20 µg/mL) for 24 to 48 hours. The cellular growth was assessed using a cell viability assay. Cellular morphology was determined by fluorescence microscopy. The gene expression profile of ABC transporters was determined by assessing a panel including 42 genes of the ABC transporter superfamily. RESULTS: We observed a significant decrease in TOV-21 G cell growth after exposure to 15 µg/mL of acetaminophen for 24 (p=0.02) and 48 hours (p=0.01), or to 20 µg/mL of ibuprofen for 48 hours (p=0.04). Assessing the morphology of TOV-21 G cells did not reveal evidence of extensive apoptosis. TOV-21 G cells had a reduced expression of the genes ABCA1, ABCC3, ABCC4, ABCD3, ABCD4 and ABCE1 within the ABC transporter superfamily. CONCLUSIONS: This study provides in vitro evidence of inhibitory effects of growth in therapeutic concentrations of acetaminophen and ibuprofen on TOV-21 G cells. Additionally, TOV-21 G cells presented a reduced expression of the ABCA1, ABCC3, ABCC4, ABCD3, ABCD4 and ABCE1 transporters.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Ibuprofeno / Neoplasias Epiteliais e Glandulares / Transportadores de Cassetes de Ligação de ATP / Proliferação de Células / Transcriptoma / Acetaminofen Limite: Female / Humans Idioma: En Revista: Rev Bras Ginecol Obstet Assunto da revista: GINECOLOGIA / OBSTETRICIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Ibuprofeno / Neoplasias Epiteliais e Glandulares / Transportadores de Cassetes de Ligação de ATP / Proliferação de Células / Transcriptoma / Acetaminofen Limite: Female / Humans Idioma: En Revista: Rev Bras Ginecol Obstet Assunto da revista: GINECOLOGIA / OBSTETRICIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil