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DNA Methylation and Hydroxymethylation Levels in Relation to Two Weight Loss Strategies: Energy-Restricted Diet or Bariatric Surgery.
Nicoletti, Carolina Ferreira; Nonino, Carla Barbosa; de Oliveira, Bruno Affonso Parenti; Pinhel, Marcela Augusta de Souza; Mansego, Maria Luisa; Milagro, Fermin Ignacio; Zulet, Maria Angeles; Martinez, José Alfredo.
Afiliação
  • Nicoletti CF; Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. carol_nicolettif@yahoo.com.br.
  • Nonino CB; Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. carla@fmrp.usp.br.
  • de Oliveira BA; Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. bruno_parenti@hotmail.com.
  • Pinhel MA; Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. marcelapinhel@yahoo.com.br.
  • Mansego ML; Department of Nutrition, Food Science and Physiology, University of Navarra, c/Irunlarrea 1, 31008, Pamplona, Spain. mlmansego@unav.es.
  • Milagro FI; Department of Nutrition, Food Science and Physiology, University of Navarra, c/Irunlarrea 1, 31008, Pamplona, Spain. fmilagro@unav.es.
  • Zulet MA; Centre for Nutrition Research, University of Navarra, Pamplona, Spain. fmilagro@unav.es.
  • Martinez JA; CIBERobn Fisiología Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain. fmilagro@unav.es.
Obes Surg ; 26(3): 603-11, 2016 Mar.
Article em En | MEDLINE | ID: mdl-26198618
BACKGROUND: Weight loss can be influenced by genetic factors and epigenetic mechanisms that participate in the regulation of body weight. This study aimed to investigate whether the weight loss induced by two different obesity treatments (energy restriction or bariatric surgery) may affect global DNA methylation (LINE-1) and hydroxymethylation profile, as well as the methylation patterns in inflammatory genes. METHODS: This study encompassed women from three differents groups: 1. control group (n = 9), normal weight individuals; 2. energy restriction group (n = 22), obese patients following an energy-restricted Mediterranean-based dietary treatment (RESMENA); and 3. bariatric surgery group (n = 14), obese patients underwent a hypocaloric diet followed by bariatric surgery. Anthropometric measurements and 12-h fasting blood samples were collected before the interventions and after 6 months. Lipid and glucose biomarkers, global hydroxymethylation (by ELISA), LINE-1, SERPINE-1, and IL-6 (by MS-HRM) methylation levels were assessed in all participants. RESULTS: Baseline LINE-1 methylation was associated with serum glucose levels whereas baseline hydroxymethylation was associated with BMI, waist circumference, total cholesterol, and triglycerides. LINE-1 and SERPINE-1 methylation levels did not change after weight loss, whereas IL-6 methylation increased after energy restriction and decreased in the bariatric surgery group. An association between SERPINE-1 methylation and weight loss responses was found. CONCLUSIONS: Global DNA methylation and hydroxymethylation might be biomarkers for obesity and associated comorbidities. Depending on the obesity treatment (diet or surgery), the DNA methylation patterns behave differently. Baseline SERPINE-1 methylation may be a predictor of weight loss values after bariatric surgery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Marcadores Genéticos / Metilação de DNA / Obesidade Tipo de estudo: Prognostic_studies Limite: Adult / Female / Humans / Middle aged Idioma: En Revista: Obes Surg Assunto da revista: METABOLISMO Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Marcadores Genéticos / Metilação de DNA / Obesidade Tipo de estudo: Prognostic_studies Limite: Adult / Female / Humans / Middle aged Idioma: En Revista: Obes Surg Assunto da revista: METABOLISMO Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos