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Observed impacts of insulin therapy on callus cell transforming growth factor-beta 1 expression in diabetic rats.
Wang, L X; Jiang, H L; Du, S L.
Afiliação
  • Wang LX; Central Sterile Supply Department, Liao Cheng People's Hospital, Liao Cheng, Shandong, China.
  • Jiang HL; Intensive Care Unit, Liao Cheng People's Hospital, Liao Cheng, Shandong, China.
  • Du SL; Department of Orthopedics, Liao Cheng People's Hospital, Shandong, China ShunleiDu@163.com.
Genet Mol Res ; 14(2): 5076-84, 2015 May 12.
Article em En | MEDLINE | ID: mdl-26125699
The expression of transforming growth factor-beta 1 (TGF-ß1) inside the callus cells of diabetic rats and the impact of insulin therapy on its expression and biomechanics was investigated. The rats were randomly divided as follows: an insulin therapy group (IT), a diabetic model group (DM), and a non-diabetic control group (NC). Bone specimens from each group were extracted at different times for immunohistochemical observation of the expression of TGF-ß1. Concurrently, the destruction torque and torsional stiffness were detected at different times. One to four weeks after fracture, TGF-ß1 was widely expressed in fractured callus cells and periosteal proliferating cells, while the expression inside diabetic cells was significantly reduced. The expression of TGF-ß1 decreased over the first 68 weeks, and the mature bone cells never expressed TGF-ß1. The destruction torque (Nm) detected in the 6th week revealed that there was a statistically significant difference between the DM, NC, and IT groups (P < 0.01). In conclusion, TGF-ß1 expression was significantly reduced inside the callus cells of diabetic rats. Insulin therapy increased TGF-ß1 expression inside the callus cells of diabetic rats and improved the biomechanical characteristics of the callus.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fraturas da Tíbia / Calo Ósseo / Diabetes Mellitus Experimental / Fator de Crescimento Transformador beta1 / Hipoglicemiantes / Insulina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fraturas da Tíbia / Calo Ósseo / Diabetes Mellitus Experimental / Fator de Crescimento Transformador beta1 / Hipoglicemiantes / Insulina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil