[Infectivity of promastigotes in stationary phase of Leishmania (viannia) braziliensis and leishmania (viannia) peruviana, In cell line dh82]. / Capacidad infectiva de promastigotes en fase estacionaria de leishmania (viannia) braziliensis y leishmania (viannia) peruviana, en línea celular dh82.
Rev Peru Med Exp Salud Publica
; 32(1): 33-40, 2015.
Article
em Es
| MEDLINE
| ID: mdl-26102103
OBJECTIVES: To determine the infectivity of promastigotes of Leishmania (V.) peruviana and Leishmania (V.) braziliensis in monocyte-macrophage cell line DH82 of Canis familiaris. MATERIALS AND METHODS: Was conducted a experimental study during the months of january to december 2013. Were used strains of Leishmania were used (V.) braziliensis MHOM/PE/84/LC53 and Leishmania (V.) peruviana MHOM/PE/84/LC26. The cell line was infected with stationary phase promastigotes and infectivity was determined as the product of percent infected macrophages average amastigotes per macrophage observed in epifluorescence microscope. RESULTS: 13% of metacyclic forms to Leishmania (V.) braziliensis corresponded to 17.5 days post inoculation and Leishmania (V.) peruviana a percentage of 9.5% on the day 14.5. No significant difference was found between infectivity of stationary phase promastigotes of both species. CONCLUSIONS: It is recommended assess the infectivity of metacyclic promastigotes peruviana strains of Leishmania (V.) and Leishmania (V.) braziliensis cell lines in order to determine the most appropriate model in vitro infection, allowing leishmanicidas make the drug more effective susceptibility studies for disease control.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leishmania braziliensis
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
Es
Revista:
Rev Peru Med Exp Salud Publica
Assunto da revista:
SAUDE PUBLICA
Ano de publicação:
2015
Tipo de documento:
Article
País de publicação:
Peru