Frequency of human platelet antigens (HPA)-1, -2, -5 and -15 in Brazilian blood donors and establishment of a panel of HPA-typed donors.
Transfus Med
; 25(3): 189-94, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-26033262
OBJECTIVES: The aim of this study is to keep a record of regular human platelet antigens (HPA)-typed blood donors and to compare their allele frequencies with those reported in other populations. BACKGROUND: HPA are polymorphisms expressed on platelet membrane glycoproteins. They can generate an immune response leading to platelet alloimmunisation that may show clinical manifestations, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet refractoriness. Platelet alloimmunisation is not uncommon, therefore, for an optimum management, it is advantageous to establish a panel of typed platelets to help matched platelets selection in HPA-alloimmunised patients transfusion. METHODS: The polymerase chain reaction (PCR)-allele-specific primers and PCR-restriction fragment length polymorphism methods were used to determine the genotypes of HPA-1, -2, -5 and -15 systems of 337 blood donors. RESULTS: The three genotypes (AA, AB and BB) were found in all HPA systems analysed, and the most frequent genotypes were AA for HPA-1, -2 and -5 systems (mean: 0·732) and AB for HPA-15 system (mean: 0·523). Allele frequencies were 0·148, 0·155, 0·140 and 0·430 for HPA-1b, -2b, -5b and -15b, respectively, and they were similar to those found in Caucasian populations, especially for HPA-1. However, the B allele was more frequent in all HPA systems when compared with Amazon Indians, and the frequency of the B allele in our study was higher in HPA-1 and -15 systems and lower in HPA-2 and -5 systems in comparison with sub-Saharan African populations. CONCLUSIONS: A record of HPA-typed donors would enable rapid identification and selection of donors when HPA-compatible platelets are required for transfusion.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doadores de Sangue
/
Polimorfismo de Fragmento de Restrição
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Antígenos de Plaquetas Humanas
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Alelos
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Técnicas de Genotipagem
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Frequência do Gene
Limite:
Female
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Humans
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Male
País/Região como assunto:
America do sul
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Brasil
Idioma:
En
Revista:
Transfus Med
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de publicação:
Reino Unido