Your browser doesn't support javascript.
loading
Periapical Lesions Decrease Insulin Signaling in Rat Skeletal Muscle.
Astolphi, Rafael Dias; Curbete, Mariane Machado; Chiba, Fernando Yamamoto; Cintra, Luciano Tavares Angelo; Ervolino, Edilson; da Mota, Max Sander de Oliveira; Antoniali, Cristina; Garbin, Cléa Adas Saliba; Sumida, Doris Hissako.
Afiliação
  • Astolphi RD; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
  • Curbete MM; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
  • Chiba FY; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
  • Cintra LT; Department of Endodontics, Araçatuba Dental School, Araçatuba, São Paulo, Brazil.
  • Ervolino E; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
  • da Mota MS; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
  • Antoniali C; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
  • Garbin CA; Department of Child and Social Dentistry, Araçatuba Dental School, UNESP, Araçatuba, São Paulo, Brazil.
  • Sumida DH; Department of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: dorishs@foa.unesp.br.
J Endod ; 41(8): 1305-10, 2015 Aug.
Article em En | MEDLINE | ID: mdl-26027876
INTRODUCTION: Serum inflammatory cytokines derived from oral inflammation are associated with decreased insulin signaling (IS) and insulin resistance, which is a major risk factor for type 2 diabetes mellitus. This study aimed to investigate IS in the liver and skeletal muscle (SM) and disorders related to the serum lipid profile and glucose and insulin levels of nondiabetic rats with induced chronic periapical lesions (PLs). METHODS: Twenty-eight Wistar rats were divided into control and PL groups. PLs were induced by exposing the pulpal tissue to the oral environment. Experiments were conducted in both groups 30 days after pulp exposure. Maxillae were processed for histopathological analysis. IS was evaluated according to insulin receptor substrate (pp185-insulin receptor substrate 1 [IRS-1]/insulin receptor substrate 2 [IRS-2]) tyrosine phosphorylation status, IRS-1 serine phosphorylation status, and IRS-1 and IRS-2 content in the liver and SM by Western blotting. Serum total cholesterol, triglyceride, glucose, and insulin levels were measured enzymatically using a commercial kit. RESULTS: PL rats showed reduced pp185 P-Tyr and increased IRS-1 serine phosphorylation status in the SM but no change in the liver after insulin stimulation. No significant changes in IRS-1 and IRS-2 content, serum total cholesterol, triglyceride, glucose or insulin levels were noted. CONCLUSIONS: PLs are associated with decreased insulin signaling in the SM of rats. Because a decrease in insulin signaling is associated with insulin resistance, our results emphasize the importance of preventing local inflammatory diseases such as PLs to prevent alterations in IS in muscle.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Músculo Esquelético / Polpa Dentária / Proteínas Substratos do Receptor de Insulina Tipo de estudo: Etiology_studies / Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Endod Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Músculo Esquelético / Polpa Dentária / Proteínas Substratos do Receptor de Insulina Tipo de estudo: Etiology_studies / Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: J Endod Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos