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Study of the relationship between mononuclear inflammatory infiltrate and Ki-67 and basement membrane and extracellular matrix protein expression in radicular cysts.
Mourão, R V C; Júnior, E C Pinheiro; Barros Silva, P G; Turatti, E; Mota, M R L; Alves, A P N N.
Afiliação
  • Mourão RV; Department of Pathology and Forensic Medicine, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
  • Júnior EC; Division of Endodontics, School of Dentistry, Fortaleza University, Fortaleza, Brazil.
  • Barros Silva PG; Division of Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
  • Turatti E; Division of Oral Pathology, School of Dentistry, Fortaleza University, Fortaleza, Brazil.
  • Mota MR; Division of Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
  • Alves AP; Division of Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
Int Endod J ; 49(5): 447-53, 2016 May.
Article em En | MEDLINE | ID: mdl-26011468
AIM: To evaluate the relationship between mononuclear inflammatory infiltrate and the expression of a proliferative immunomarker (Ki-67) as well as to evaluate basement membrane and extracellular matrix proteins (laminin and collagen type IV) in radicular cysts and dentigerous cysts (DC). METHODOLOGY: Immunohistochemical analyses were performed in heavily inflamed radicular cysts (HIRC), slightly inflamed radicular cysts (SIRC) and DC (n = 20) using Ki-67 (Dako(®) , 1 : 50), anticollagen type IV (DBS(®) , 1 : 40) and antilaminin (DBS(®) , 1 : 20). The data were analysed using anova/Tukey's test (Ki-67) and Kruskal-Wallis/Dunn's test (collagen type IV and laminin) (P < 0.05). RESULTS: The immunoexpression of Ki-67 was significantly greater in the SIRC group compared with the HIRC and DC (P = 0.0040). Likewise, the immunoexpression of collagen type IV in the basement membrane of the SIRC group was significantly more continuous (P = 0.0475) than in the HIRC group. DC had significantly less collagen type IV in extracellular matrix immunoexpression than HIRC and SIRC (P = 0.0246). Laminin was absent in the basement membrane in the SIRC and DC groups, and the extracellular matrix of the HIRC was weak and punctate. CONCLUSION: The presence of inflammatory factors in the radicular cyst wall modified the expression of proliferation factors in the epithelial lining and the expression of collagen type IV and laminin in the basement membrane, but did not modify extracellular matrix behaviour in radicular cysts.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cisto Radicular / Proteínas da Matriz Extracelular / Antígeno Ki-67 Limite: Humans Idioma: En Revista: Int Endod J Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cisto Radicular / Proteínas da Matriz Extracelular / Antígeno Ki-67 Limite: Humans Idioma: En Revista: Int Endod J Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido