[Morphological and molecular characterization of isolates of Macrophomina phaseolina associated with sugarcane in Mexico]. / Caracterización morfológica y molecular de aislados de Macrophomina phaseolina asociados a caña de azúcar en México.
Rev Argent Microbiol
; 47(2): 143-7, 2015.
Article
em Es
| MEDLINE
| ID: mdl-25979147
Charcoal rot caused by Macrophomina phaseolina is an important disease of sugarcane in Mexico. This study was carried out to characterize isolates of M. phaseolina obtained from sugarcane by the combination of morphological and molecular analyses. The morphological characterization of 10 isolates was performed using scanning electron microscopy and light microscopy. To confirm the morphological identification, rDNA from two representative isolates was extracted, and the internal transcribed spacer (ITS) region was amplified by polymerase chain reaction and sequenced using specific primers MpKF1 and MpKR1. Based on their morphological characteristics, all isolates were identified as M. phaseolina. Moreover, the analysis of two ITS sequences showed 100% similarity with the M. phaseolina sequences deposited in the GenBank. To our knowledge, this is the first study in the world aimed at characterizing isolates of M. phaseolina obtained from sugarcane.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Saccharum
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
País/Região como assunto:
Mexico
Idioma:
Es
Revista:
Rev Argent Microbiol
Ano de publicação:
2015
Tipo de documento:
Article
País de publicação:
Argentina