Effects of sulfated and non-sulfated ß-glucan extracted from Agaricus brasiliensis in breast adenocarcinoma cells - MCF-7.
Toxicol Mech Methods
; 25(9): 672-9, 2015.
Article
em En
| MEDLINE
| ID: mdl-25970150
The ß-glucans (ß-G) are polysaccharides produced by various organisms, and sulfation of ß-G renders them more soluble. With the objective to assess the effects of sulfated and non-sulfated ß-G extracted from Agaricus brasiliensis in MCF-7 cells, assays were used to evaluate cytotoxicity, genotoxicity, cell proliferation and mRNA expression. The sulfated and non-sulfated ß-G showed dose-dependent cytotoxicity at concentrations of 5 and 10 µg/mL, by the MTT assay. However, only cytotoxicity was observed after 24 h by the Red Neutral test for sulfated ß-G, with no genotoxicity for either ß-G in comet assay. Proliferation was decreased only at 72 h at a concentration of 100 µg/mL of sulfated ß-G. Treatment with 5 µg/mL of sulfated ß-G for 6 h reduced the expression of pro-apoptotic genes and stress signaling genes, cell cycle arrest, damage and cell migration. The 5 µg/mL of non-sulfated ß-G for 6 h reduced the expression of the stress response gene and signaling damage. These results indicate that the cytotoxicity in the MTT is not cell death, and that, in general, sulfated ß-G have greater cytotoxicity compared to non-sulfated ß-G.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Agaricus
/
Beta-Glucanas
/
Proliferação de Células
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Polissacarídeos Fúngicos
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Antineoplásicos
Limite:
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Toxicol Mech Methods
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de publicação:
Reino Unido