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Post-transcriptional control of Amblyomin-X on secretion of vascular endothelial growth factor and expression of adhesion molecules in endothelial cells.
Drewes, C C; Dias, R Y; Branco, V G; Cavalcante, M F; Souza, J G; Abdalla, D S P; Chudzinski-Tavassi, A M; Farsky, S H P.
Afiliação
  • Drewes CC; Laboratory of Experimental Toxicology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Ave. Prof. Lineu Prestes 580, SP, 05503-900, São Paulo, Brazil.
  • Dias RY; Laboratory of Experimental Toxicology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Ave. Prof. Lineu Prestes 580, SP, 05503-900, São Paulo, Brazil.
  • Branco VG; Biochemistry and Biophysics Laboratory, Butantan Institute, Ave. Vital Brasil 1500, SP, 05503-900, São Paulo, Brazil.
  • Cavalcante MF; Laboratory of Biochemistry, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Ave. Prof. Lineu Prestes 580, SP, 05503-900, São Paulo, Brazil.
  • Souza JG; Biochemistry and Biophysics Laboratory, Butantan Institute, Ave. Vital Brasil 1500, SP, 05503-900, São Paulo, Brazil.
  • Abdalla DS; Laboratory of Biochemistry, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Ave. Prof. Lineu Prestes 580, SP, 05503-900, São Paulo, Brazil.
  • Chudzinski-Tavassi AM; Biochemistry and Biophysics Laboratory, Butantan Institute, Ave. Vital Brasil 1500, SP, 05503-900, São Paulo, Brazil.
  • Farsky SH; Laboratory of Experimental Toxicology, Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Ave. Prof. Lineu Prestes 580, SP, 05503-900, São Paulo, Brazil. Electronic address: sfarsky@usp.br.
Toxicon ; 101: 1-10, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25912945
Angiogenesis is a pivotal process of homeostasis and tissue repair, but it also favours neovascularisation syndromes and cancer nutrition. The chemical mediation of angiogenesis is complex, involving a balance between serine proteases and their inhibitors. We addressed the mechanisms of action of a Kunitz serine protease inhibitor (KPI) on spontaneous angiogenesis, using Amblyomin-X, a KPI designed from the cDNA library of the Amblyomma cajennense tick. Amblyomin-X treatment (10-1000 ng/10 µL; each 48 h; 3 times) reduced the number of vessels in the subcutaneous dorsal tissue of male Swiss mice, as measured by intravital microscopy, haematoxylin-eosin staining, and PECAM-1 immunofluorescence labeling. Incubation of Amblyomin-X with t-End endothelial cells, a murine endothelial microvascular lineage, did not alter cell proliferation, cell-cycle phases, necrosis and apoptosis, and the production of nitric oxide and prostaglandin E2. Nevertheless, Amblyomin-X treatment reduced t-End migration and adhesion to Matrigel(®), and inhibited the VEGF-A secretion and VCAM-1 and ß3 integrin expressions by posttranscriptional pathways. Together, data herein outline novel posttranscriptional mechanisms of KPIs on endothelial cells during angiogenesis and point out the possible application of Amblyomin-X as a local inhibitor to undesired neovascularisation process.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas e Peptídeos Salivares / Adesão Celular / Processamento de Proteína Pós-Traducional / Regulação da Expressão Gênica / Células Endoteliais / Fator A de Crescimento do Endotélio Vascular Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas e Peptídeos Salivares / Adesão Celular / Processamento de Proteína Pós-Traducional / Regulação da Expressão Gênica / Células Endoteliais / Fator A de Crescimento do Endotélio Vascular Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido