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PIAS-like protein Zimp7 is required for the restriction of the zebrafish organizer and mesoderm development.
Moreno-Ayala, Roberto; Schnabel, Denhí; Salas-Vidal, Enrique; Lomelí, Hilda.
Afiliação
  • Moreno-Ayala R; Departamento de Genética del Desarrollo y Fisiología Molecular Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico.
  • Schnabel D; Departamento de Genética del Desarrollo y Fisiología Molecular Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico.
  • Salas-Vidal E; Departamento de Genética del Desarrollo y Fisiología Molecular Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico.
  • Lomelí H; Departamento de Genética del Desarrollo y Fisiología Molecular Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico. Electronic address: hilda@ibt.unam.mx.
Dev Biol ; 403(1): 89-100, 2015 Jul 01.
Article em En | MEDLINE | ID: mdl-25912688
The Zmiz2 (Zimp7) protein and its homolog Zmiz1 (Zimp10) were initially identified in humans as androgen receptor co-activators. Sequence analysis revealed the presence of an SP-RING/Miz domain, which is highly conserved in members of the PIAS family and confers SUMO-conjugating activity. Zimp7 has been shown to interact with components of the Wnt/ß-Catenin signaling pathway and with Brg1 and BAF57, components of the ATP-dependent mammalian SWI/SNF-like BAF chromatin-remodeling complexes. In this work, we analyze the role of zygotic Zimp7 in zebrafish development. We describe evidence indicating that Zimp7 is required for mesoderm development and dorsoventral patterning. Morpholino-mediated reduction of zygotic Zimp7 produced axial mesodermal defects that were preceded by up-regulation of organizer genes such as bozozok, goosecoid and floating head at the onset of gastrulation and by down-regulation of the ventral markers vox, vent and eve1 indicating loss of the ventrolateral mesoderm. Consistently, embryos overexpressing zimp7 RNA exhibited midline defects such as loss of forebrain and cyclopia accompanied by transcriptional changes directly opposite of those found in the morphants. In addition, the patterning of ventralized embryos produced by the overexpression of vox and vent was restored by a reduction of Zimp7 activity. Altogether, our findings indicate that Zimp7 is involved in transcriptional regulation of factors that are essential for patterning in the dorsoventral axis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Dedos de Zinco / Regulação da Expressão Gênica no Desenvolvimento / Padronização Corporal / Organizadores Embrionários / Proteínas de Peixe-Zebra / Proteínas Inibidoras de STAT Ativados Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Dev Biol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Dedos de Zinco / Regulação da Expressão Gênica no Desenvolvimento / Padronização Corporal / Organizadores Embrionários / Proteínas de Peixe-Zebra / Proteínas Inibidoras de STAT Ativados Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Dev Biol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos