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Genomic identification and quantification of microbial species adhering to toothbrush bristles after disinfection: A cross-over study.
do Nascimento, Cássio; Trinca, Nayara Nascimento; Pita, Murillo Sucena; Pedrazzi, Vinícius.
Afiliação
  • do Nascimento C; Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto, SP 14040-904, Brazil.
  • Trinca NN; Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto, SP 14040-904, Brazil.
  • Pita MS; Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto, SP 14040-904, Brazil.
  • Pedrazzi V; Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto, SP 14040-904, Brazil. Electronic address: pedrazzi@forp.usp.br.
Arch Oral Biol ; 60(7): 1039-47, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25912552
PURPOSE: The aim of this clinical investigation was to identify and quantify the microbial species adhering to toothbrush bristles after controlled brushing and storage in different antimicrobial agents. METHODS: Sixteen healthy participants were enrolled in this study and randomly submitted to 4 interventions in a cross-over design: brushing and toothbrush storage in (I) Periogard/(II) Periobio (Chlorhexidine gluconate 0.12%), (III) Cepacol (cetylpyridinium chloride 0.05%) and (IV) distilled water (positive control). Thirty-eight bacterial species including putative pathogens and 5 Candida spp. were assessed by Checkerboard DNA-DNA hybridization. RESULTS: The results of the study have shown a striking reduction of the total microbial counts, including bacteria and Candida spp., on the toothbrush bristles after storage in cetylpyridinium chloride 0.05% (p < 0.0001). Chlorhexidine gluconate 0.12% showed no differences on the total bacterial count when compared to distilled water (p > 0.05). Cetylpyridinium chloride solution also presented the lowest genome counts and frequency of detection for individual target species; distilled water showed the highest individual genome counts (p < 0.05). Potential pathogenic species were recorded in moderate to high levels for chlorhexidine gluconate and distilled water. CONCLUSION: Cetylpyridinium chloride 0.05% was the most effective storage solution in the reduction of total and individual microbial counts, including pathogenic species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Escovação Dentária / Candida / Cetilpiridínio / Clorexidina / Desinfecção / Genômica / Anti-Infecciosos Locais Tipo de estudo: Clinical_trials / Diagnostic_studies Limite: Adult / Female / Humans / Male Idioma: En Revista: Arch Oral Biol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Escovação Dentária / Candida / Cetilpiridínio / Clorexidina / Desinfecção / Genômica / Anti-Infecciosos Locais Tipo de estudo: Clinical_trials / Diagnostic_studies Limite: Adult / Female / Humans / Male Idioma: En Revista: Arch Oral Biol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido