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Uncoupling protein-2 mRNA expression in mice subjected to intermittent hypoxia.
Vieira, Luciana Rodrigues; Martinez, Denis; Forgiarini, Luiz Felipe; Rosa, Darlan Pase da; Muñoz, Gustavo Alfredo Ochs de; Fagundes, Micheli; Martins, Emerson Ferreira; Montanari, Carolina Caruccio; Fiori, Cintia Zappe.
Afiliação
  • Vieira LR; Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Martinez D; School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Forgiarini LF; Faculdade Anhanguera de Pelotas, Pelotas, Brazil.
  • Rosa DP; Lutheran University of Brazil, Canoas, Brazil.
  • Muñoz GA; Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Fagundes M; Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Martins EF; Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Montanari CC; Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Fiori CZ; Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
J Bras Pneumol ; 41(2): 167-74, 2015.
Article em En, Pt | MEDLINE | ID: mdl-25909153
OBJECTIVE: To investigate the effect of intermittent hypoxia-a model of obstructive sleep apnea (OSA)-on pancreatic expression of uncoupling protein-2 (UCP2), as well as on glycemic and lipid profiles, in C57BL mice. METHODS: For 8 h/day over a 35-day period, male C57BL mice were exposed to intermittent hypoxia (hypoxia group) or to a sham procedure (normoxia group). The intermittent hypoxia condition involved exposing mice to an atmosphere of 92% N and 8% CO2 for 30 s, progressively reducing the fraction of inspired oxygen to 8 ± 1%, after which they were exposed to room air for 30 s and the cycle was repeated (480 cycles over the 8-h experimental period). Pancreases were dissected to isolate the islets. Real-time PCR was performed with TaqMan assays. RESULTS: Expression of UCP2 mRNA in pancreatic islets was 20% higher in the normoxia group than in the hypoxia group (p = 0.11). Fasting serum insulin was higher in the hypoxia group than in the normoxia group (p = 0.01). The homeostasis model assessment of insulin resistance indicated that, in comparison with the control mice, the mice exposed to intermittent hypoxia showed 15% lower insulin resistance (p = 0.09) and 21% higher pancreatic ß-cell function (p = 0.01). Immunohistochemical staining of the islets showed no significant differences between the two groups in terms of the area or intensity of α- and ß-cell staining for insulin and glucagon. CONCLUSIONS: To our knowledge, this is the first report of the effect of intermittent hypoxia on UCP2 expression. Our findings suggest that UCP2 regulates insulin production in OSA. Further study of the role that UCP2 plays in the glycemic control of OSA patients is warranted.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Ilhotas Pancreáticas / Apneia Obstrutiva do Sono / Proteínas Mitocondriais / Canais Iônicos / Hipóxia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En / Pt Revista: J Bras Pneumol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Ilhotas Pancreáticas / Apneia Obstrutiva do Sono / Proteínas Mitocondriais / Canais Iônicos / Hipóxia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En / Pt Revista: J Bras Pneumol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil