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Cytotoxicity evaluation of a copaiba oil-based root canal sealer compared to three commonly used sealers in endodontics.
Garrido, Angela Delfina Bittencourt; de Cara, Sueli Patricia Harumi Miyagi; Marques, Marcia Martins; Sponchiado, Emílio Carlos; Garcia, Lucas da Fonseca Roberti; de Sousa-Neto, Manoel Damião.
Afiliação
  • Garrido AD; Department of Endodontics, School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • de Cara SP; Department of Dentistry, School of Dentistry, University of São Paulo, Brazil.
  • Marques MM; Department of Dentistry, School of Dentistry, University of São Paulo, Brazil.
  • Sponchiado EC; Department of Endodontics, School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • Garcia Lda F; Department of Dental Materials and Prosthodontics, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • de Sousa-Neto MD; Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Dent Res J (Isfahan) ; 12(2): 121-6, 2015.
Article em En | MEDLINE | ID: mdl-25878676
BACKGROUND: The constant development of new root canal sealers has allowed the solution of a large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as filling materials. The aim of this study was to evaluate the cytotoxic effect of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells. MATERIALS AND METHODS: The experimental groups were formed according to the culture medium conditioned with the tested sealers, as follows: Control group (CG) (culture medium without conditioning); Sealer 26 (S26) - culture medium + S26; Endofill (EF) - culture medium + EF; AH Plus (AHP) - culture medium + AHP; and BS - culture medium + BS (Copaiba oil-based sealer). The conditioned culture medium was placed in contact with 2 × 10(4) cells cultivated on 60 mm diameter Petri dishes for 24 h. Then, hemocytometer count was performed to evaluate cellular viability, using Trypan Blue assay. The normal distribution of data was tested by the Kolmogorov-Smirnov test and the values obtained for cellular viability were statistically analyzed (1-way ANOVA, Tukey's test - P < 0.05), with a significance level of 5%. RESULTS: S26, EF and AHP presented decreased cellular viability considerably, with statistical significance compared with CG (P < 0.05). BS maintained cellular viability similar to CG (P > 0.05). CONCLUSION: The Copaiba oil-based root canal sealer presented promising results in terms of cytotoxicity which indicated its usefulness as a root canal sealer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Dent Res J (Isfahan) Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irã
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Dent Res J (Isfahan) Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irã