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Comparative effects of photodynamic therapy mediated by curcumin on standard and clinical isolate of Streptococcus mutans.
Tonon, Caroline C; Paschoal, Marco Aurélio; Correia, Marilia; Spolidório, Denise M P; Bagnato, Vanderlei S; Giusti, Juçaíra S M; Santos-Pinto, Lourdes.
Afiliação
  • Tonon CC; Araraquara Dental School, Universidade Estadual Paulista Araraquara, São Paulo, Brazil.
  • Paschoal MA; Master Program in Dentistry, CEUMA University, São Luís, Maranhão, Brazil.
  • Correia M; Department of Pediatric Dentistry, Araraquara Dental School Universidade Estadual Paulista, Araraquara, São Paulo, Brazil.
  • Spolidório DM; Department of Pathology and Physiology, Araraquara Dental School, Universidade Estadual Paulista, Araraquara, São Paulo, Brazil.
  • Bagnato VS; São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo, Brazil.
  • Giusti JS; São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo, Brazil.
  • Santos-Pinto L; PhD, Department of Pediatric Dentistry, Araraquara Dental School Universidade Estadual Paulista, Araraquara, São Paulo, Brazil, Rua Humaité, 1680 Araraquara, São Paulo 14801-903, Brazil Phone: 55 16 33016330, Fax: 55 16 33016328, e-mail: lspinto@foar.unesp.br.
J Contemp Dent Pract ; 16(1): 1-6, 2015 01 01.
Article em En | MEDLINE | ID: mdl-25876942
AIM: The aim of this study was investigate the effect of photodynamic therapy (PDT) using curcumin (C) as a photosensitizing agent irradiated with an LED (L) in the blue wavelength as a light source on a standard and clinical isolate of Streptococcus mutans (S. mutans) in a planktonic suspension model. MATERIALS AND METHODS: Suspensions of both strains were divided into 4 groups as follows: absence of C and L (control group: C-L-), with C and without L (C group: C+L-), absence of C with L (L group: C-L+) and presence of C and L (PDT group: C+L+). Three different concentrations of curcumin (0.75 mg/ml, 1.5 mg/ml and 3 mg/ml) and three light fluences of studied light source (24, 48 and 72 J cm(-2)) were tested. Aliquots of each studied group was plated in BHI agar and submitted to colony forming units counting (CFU/ml) and the data transformed into logarithmical scale. RESULTS: A high photoinactivation rate of more than 70% was verified to standard S. mutans strain submitted to PDT whereas the clinical isolate showed a lower sensitivity to all the associations of curcumin and LED. A slight bacterial reduction was verified to C+L- and C-L+, demonstrating no toxic effects to the isolated application of light and photosensitizer to both S. mutans strains tested. CONCLUSION: Photodynamic therapy using a combination of curcumin and blue LED presented a substantial antimicrobial effect on S. mutans standard strain in a planktonic suspension model with a less pronounced effect on its clinical isolate counterparts due to resistance to this alternative approach. CLINICAL SIGNIFICANCE: Alternative antimicrobial approaches, as photodynamic therapy, should be encouraged due to optimal results against cariogenic bacteria aiming to prevent or treat dental caries.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Streptococcus mutans / Fármacos Fotossensibilizantes / Curcumina Limite: Humans Idioma: En Revista: J Contemp Dent Pract Assunto da revista: ODONTOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Índia
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Streptococcus mutans / Fármacos Fotossensibilizantes / Curcumina Limite: Humans Idioma: En Revista: J Contemp Dent Pract Assunto da revista: ODONTOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Índia