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Molecular identity of ramie germplasms using simple sequence repeat markers.
Luan, M B; Chen, B F; Zou, Z Z; Zhu, J J; Wang, X F; Xu, Y; Sun, Z M; Chen, J H.
Afiliação
  • Luan MB; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China.
  • Chen BF; Department of Medical Biology, North Sichuan Medical College, Sichuan Nanchong, China.
  • Zou ZZ; Yiyang Medical School, Yiyang, China.
  • Zhu JJ; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China.
  • Wang XF; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China.
  • Xu Y; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China.
  • Sun ZM; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China.
  • Chen JH; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China luanmingbao2002@126.com.
Genet Mol Res ; 14(1): 2302-11, 2015 Mar 27.
Article em En | MEDLINE | ID: mdl-25867376
DNA identity is highly effective and efficient for distinguishing crop varieties regardless of their phenotypic similarities. To establish DNA identity in ramie, 21 simple sequence repeat primers were amplified in 108 accessions of domestic and exotic ramie germplasms. Sixty polymorphic bands were obtained, with an average of 2.9 bands per locus and 2-8 band types per primer locus (average of 5.19 band types). The Simpson's diversity index of the 21 simple sequence repeat loci ranged from 0.158 to 0.808 with an average of 0.612. There was large difference in the specific index in the germplasm tested, from 44.082 to 218.163, with an average of 83.620. Based on allele band type, 8 primer pairs were selected for DNA fingerprinting of the 108 genotypes. The combination of the 8 primer pairs were found to be very effective for distinguishing these genotypes, indicating that they can be used in the molecular DNA identity of ramie.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Sementes / Repetições de Microssatélites / Boehmeria Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Sementes / Repetições de Microssatélites / Boehmeria Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil