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Immunomodulatory and Antileishmanial Activity of Phenylpropanoid Dimers Isolated from Nectandra leucantha.
da Costa-Silva, Thais Alves; Grecco, Simone S; de Sousa, Fernanda S; Lago, João Henrique G; Martins, Euder G A; Terrazas, César A; Varikuti, Sanjay; Owens, Katherine L; Beverley, Stephen M; Satoskar, Abhay R; Tempone, Andre G.
Afiliação
  • da Costa-Silva TA; †Center for Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil.
  • Grecco SS; ‡Center of Natural Sciences and Humanities, Federal University of ABC, São Paulo, Brazil.
  • de Sousa FS; §Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, São Paulo, Brazil.
  • Lago JH; §Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, São Paulo, Brazil.
  • Martins EG; ⊥Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Terrazas CA; ∥Departments of Pathology and Microbiology, Ohio State University, Columbus, Ohio, United States.
  • Varikuti S; ∥Departments of Pathology and Microbiology, Ohio State University, Columbus, Ohio, United States.
  • Owens KL; ∇Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States.
  • Beverley SM; ∇Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States.
  • Satoskar AR; ∥Departments of Pathology and Microbiology, Ohio State University, Columbus, Ohio, United States.
  • Tempone AG; †Center for Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil.
J Nat Prod ; 78(4): 653-7, 2015 Apr 24.
Article em En | MEDLINE | ID: mdl-25835647
Three phenylpropanoid dimers (1-3) including two new metabolites were isolated from the extract of the twigs of Nectandra leucantha using antileishmanial bioassay-guided fractionation. The in vitro antiparasitic activity of the isolated compounds against Leishmania donovani parasites and mammalian cytotoxicity and immunomodulatory effects were evaluated. Compounds 1-3 were effective against the intracellular amastigotes within macrophages, with IC50 values of 26.7, 17.8, and 101.9 µM, respectively. The mammalian cytotoxicity, given by the 50% cytotoxic concentration (CC50), was evaluated against peritoneal macrophages. Compounds 1 and 3 were not toxic up to 290 µM, whereas compound 2 demonstrated a CC50 value of 111.2 µM. Compounds 1-3 also suppressed production of disease exacerbatory cytokines IL-6 and IL-10 but had minimal effect on nitric oxide production in L. donovani-infected macrophages, indicating that antileishmanial activity of these compounds is mediated via an NO-independent mechanism. Therefore, these new natural products could represent promising scaffolds for drug design studies for leishmaniasis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilpropionatos / Leishmaniose / Lauraceae / Fatores Imunológicos / Anisóis / Antiprotozoários Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Nat Prod Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilpropionatos / Leishmaniose / Lauraceae / Fatores Imunológicos / Anisóis / Antiprotozoários Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Nat Prod Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos