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Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning.
Cui, S Q; Wang, Q; Zheng, Y; Xiao, B; Sun, H W; Gu, X L; Zhang, Y C; Fu, C H; Dong, P X; Wang, X M.
Afiliação
  • Cui SQ; China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.
  • Wang Q; Department of Physiology, Capital Medical University, Beijing, China.
  • Zheng Y; Department of Physiology, Capital Medical University, Beijing, China.
  • Xiao B; Department of Physiology, Capital Medical University, Beijing, China.
  • Sun HW; China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.
  • Gu XL; China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.
  • Zhang YC; China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.
  • Fu CH; China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.
  • Dong PX; China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.
  • Wang XM; Department of Physiology, Capital Medical University, Beijing, China.
Braz J Med Biol Res ; 48(6): 515-22, 2015 Jun.
Article em En | MEDLINE | ID: mdl-25831201
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1ß were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1ß increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vasodilatadores / Fármacos Neuroprotetores / Aprendizagem em Labirinto / Etanol / Memória Espacial / Isoflavonas / Transtornos da Memória Tipo de estudo: Evaluation_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vasodilatadores / Fármacos Neuroprotetores / Aprendizagem em Labirinto / Etanol / Memória Espacial / Isoflavonas / Transtornos da Memória Tipo de estudo: Evaluation_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil