Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts.
Biotechnol Adv
; 33(5): 435-56, 2015.
Article
em En
| MEDLINE
| ID: mdl-25777494
In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of antibodies, the development of specific domains with affinity for some specific supports will be revised. Moreover, we will discuss the use of domains that increase the affinity for standard matrices (ionic exchangers, silicates). We will show how the control of the immobilization conditions may convert some unspecific supports in largely specific ones. The development of tailor-made heterofunctional supports as a tool to immobilize-stabilize-purify some proteins will be discussed in deep, using low concentration of adsorbent groups and a dense layer of groups able to give an intense multipoint covalent attachment. The final coupling of mutagenesis and tailor made supports will be the last part of the review.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biotecnologia
/
Enzimas Imobilizadas
Idioma:
En
Revista:
Biotechnol Adv
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Colômbia
País de publicação:
Reino Unido