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Didanosine-loaded chitosan microspheres optimized by surface-response methodology: a modified "Maximum Likelihood Classification" approach formulation for reverse transcriptase inhibitors.
Ferreira da Silva, Classius; Severino, Patrícia; Martins, Fernanda; Santana, Maria Helena A; Souto, Eliana B.
Afiliação
  • Ferreira da Silva C; Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo, Rua Arthur Riedel, 275, Diadema 09972-270, Brazil.
  • Severino P; Department of Biotechnological Processes, School of Engineering Chemical, University of Campinas, Campinas 13083-970, Brazil; University of Tiradentes and Institute of Technology and Research, Av. Murilo Dantas 300, 49010-390 Aracaju, Brazil.
  • Martins F; Department of Biotechnological Processes, School of Engineering Chemical, University of Campinas, Campinas 13083-970, Brazil.
  • Santana MH; Department of Biotechnological Processes, School of Engineering Chemical, University of Campinas, Campinas 13083-970, Brazil. Electronic address: lena@feq.unicamp.br.
  • Souto EB; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal; Center for Neuroscience and Cell Biology & Institute for Biomedical Imaging and Life Sciences (CNC-IBILI), University of Co
Biomed Pharmacother ; 70: 46-52, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25776478
Didanosine-loaded chitosan microspheres were developed applying a surface-response methodology and using a modified Maximum Likelihood Classification. The operational conditions were optimized with the aim of maintaining the active form of didanosine (ddI), which is sensitive to acid pH, and to develop a modified and mucoadhesive formulation. The loading of the drug within the chitosan microspheres was carried out by ionotropic gelation technique with sodium tripolyphosphate (TPP) as cross-linking agent and magnesium hydroxide (Mg(OH)2) to assure the stability of ddI. The optimization conditions were set using a surface-response methodology and applying the "Maximum Likelihood Classification", where the initial chitosan concentration, TPP and ddI concentration were set as the independent variables. The maximum ddI-loaded in microspheres (i.e. 1433 mg of ddI/g chitosan), was obtained with 2% (w/v) chitosan and 10% TPP. The microspheres depicted an average diameter of 11.42 µm and ddI was gradually released during 2 h in simulated enteric fluid.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Didanosina / Inibidores da Transcriptase Reversa / Quitosana / Microesferas Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Didanosina / Inibidores da Transcriptase Reversa / Quitosana / Microesferas Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: França