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Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes.
Santiago, Karina Basso; Conti, Bruno José; Murbach Teles Andrade, Bruna Fernanda; Mangabeira da Silva, Jonas Joaquim; Rogez, Hervé Louis Ghislain; Crevelin, Eduardo José; Beraldo de Moraes, Luiz Alberto; Veneziani, Rodrigo; Ambrósio, Sérgio Ricardo; Bastos, Jairo Kenupp; Sforcin, José Maurício.
Afiliação
  • Santiago KB; Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil.
  • Conti BJ; Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil.
  • Murbach Teles Andrade BF; Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil.
  • Mangabeira da Silva JJ; Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
  • Rogez HL; School of Food Engeneering, Institute of Technology, Federal University of Pará, 66095-780, Belém, PA, Brazil.
  • Crevelin EJ; Chemistry Department, School of Phylosophy, Sciences and Languages, University of São Paulo, 14040-901, Ribeirão Preto, SP, Brazil.
  • Beraldo de Moraes LA; Chemistry Department, School of Phylosophy, Sciences and Languages, University of São Paulo, 14040-901, Ribeirão Preto, SP, Brazil.
  • Veneziani R; University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, 14404-600, Franca, SP, Brazil.
  • Ambrósio SR; University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, 14404-600, Franca, SP, Brazil.
  • Bastos JK; Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
  • Sforcin JM; Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil. Electronic address: sforcin@ibb.unesp.br.
Biomed Pharmacother ; 70: 12-8, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25776472
Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunomodulatory action has not been investigated. Thus, the goal of this study was to characterize different oleoresins and to verify their action on human monocytes regarding pro- and anti-inflammatory cytokine production (TNF-α and IL-10, respectively). The chemical composition of Brazilian Copaifera reticulata, Copaifera duckey and Copaifera multijuga oleoresins was analyzed by HPLC-MS. Cell viability was assessed by MTT method after incubation of cells with Copaifera spp. Noncytotoxic concentrations of oleoresins were incubated with human monocytes from healthy donors, and cytokine production was determined by ELISA. HPLC-MS analysis for terpenes allowed the identification of six diterpene acids and one sesquiterpene acid. Oleoresins exerted no cytotoxic effects on human monocytes. All oleoresins had a similar profile: LPS-induced TNF-α production was maintained by oleoresins, while a significant inhibitory action on IL-10 production was seen. Copaifera oleoresins seemed to exert an activator profile on human monocytes without affecting cell viability. Such effect may be due to the presence of either diterpene or sesquiterpene acids; however, further studies are necessary to determine the involvement of such compounds in Copaifera immunomodulatory effects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bálsamos / Monócitos / Regulação da Expressão Gênica / Fator de Necrose Tumoral alfa / Interleucina-10 / Fabaceae Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bálsamos / Monócitos / Regulação da Expressão Gênica / Fator de Necrose Tumoral alfa / Interleucina-10 / Fabaceae Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: França