Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells.
Rev Argent Microbiol
; 47(1): 4-8, 2015.
Article
em En
| MEDLINE
| ID: mdl-25697468
Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines. To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed in the baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected for a cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developed high levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstrating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-up and was transferred to the private sector. Nowadays, it is being evaluated for registration as the first Argentinean subunit vaccine for cattle.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas
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Doenças dos Bovinos
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Vacinas de Subunidades Antigênicas
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Diarreia
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Anticorpos de Cadeia Única
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Células Apresentadoras de Antígenos
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Rev Argent Microbiol
Ano de publicação:
2015
Tipo de documento:
Article
País de publicação:
Argentina