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Metallothionein gene expression is altered in oral cancer and may predict metastasis and patient outcomes.
Brazão-Silva, Marco T; Rodrigues, Maria Fernandes S; Eisenberg, Ana Lúcia A; Dias, Fernando L; de Castro, Luciana M; Nunes, Fábio D; Faria, Paulo R; Cardoso, Sérgio V; Loyola, Adriano M; de Sousa, Suzana C O M.
Afiliação
  • Brazão-Silva MT; PhD program in Estomatology and Basic and Applied Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Rodrigues MF; Laboratory of Molecular Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Eisenberg AL; Department of Pathology, Brazilian National Cancer Institute/INCA, Rio de Janeiro, Brazil.
  • Dias FL; Department of Head and Neck Surgery, Brazilian National Cancer Institute/INCA, Rio de Janeiro, Brazil.
  • de Castro LM; National Tumor Bank, Brazilian National Cancer Institute/INCA, Rio de Janeiro, Brazil.
  • Nunes FD; PhD program in Estomatology and Basic and Applied Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Faria PR; Laboratory of Molecular Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Cardoso SV; Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Loyola AM; Department of Histology and Morphology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, Brazil.
  • de Sousa SC; Department of Oral Pathology, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Histopathology ; 67(3): 358-67, 2015 Sep.
Article em En | MEDLINE | ID: mdl-25640883
AIMS: Metallothioneins (MTs) are proteins associated with the carcinogenesis and prognosis of various tumours. Previous studies have shown their potential as biomarkers in oral squamous cell carcinoma (OSCC). Aiming to understand more clearly the function of MTs in OSCC we evaluated, for the first time, the gene expression profile of MTs in this neoplasm. MATERIALS AND RESULTS: Tissue samples from 35 cases of tongue and/or floor of mouth OSCC, paired with their corresponding non-neoplastic oral mucosa (NNOM), were retrieved (2007-09). All tissues were analysed for the following genes using TaqMan(®) reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays: MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1X, MT2A, MT3 and MT4. The expression of MT1B and MT1H was seldom detected in both OSCC and NNOM. A significant loss of MT1A, MT1X, MT3 and MT4 expression and gain of MT1F expression was observed in OSCC, compared to NNOM. Cases with MT1G down-regulation exhibited the worst prognoses. The up-regulation of MT1X was restricted to non-metastatic cases, whereas up-regulation of MT3 was related to cases with lymph node metastasis. CONCLUSIONS: Metallothionein mRNA expression is altered significantly in oral squamous cell carcinomas. The expression of MT1G, MT1X and MT3 may aid in the prognostic discrimination of OSCC cases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Carcinoma de Células Escamosas / Metalotioneína Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Histopathology Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Carcinoma de Células Escamosas / Metalotioneína Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Histopathology Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido