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Identification of reference genes and validation for gene expression studies in diverse axolotl (Ambystoma mexicanum) tissues.
Guelke, Eileen; Bucan, Vesna; Liebsch, Christina; Lazaridis, Andrea; Radtke, Christine; Vogt, Peter M; Reimers, Kerstin.
Afiliação
  • Guelke E; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
  • Bucan V; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
  • Liebsch C; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
  • Lazaridis A; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
  • Radtke C; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
  • Vogt PM; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany.
  • Reimers K; Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30659 Hannover, Germany. Electronic address: reimers.kerstin@mh-hannover.de.
Gene ; 560(1): 114-23, 2015 Apr 10.
Article em En | MEDLINE | ID: mdl-25637570
For the precise quantitative RT-PCR normalization a set of valid reference genes is obligatory. Moreover have to be taken into concern the experimental conditions as they bias the regulation of reference genes. Up till now, no reference targets have been described for the axolotl (Ambystoma mexicanum). In a search in the public database SalSite for genetic information of the axolotl we identified fourteen presumptive reference genes, eleven of which were further tested for their gene expression stability. This study characterizes the expressional patterns of 11 putative endogenous control genes during axolotl limb regeneration and in an axolotl tissue panel. All 11 reference genes showed variable expression. Strikingly, ACTB was to be found most stable expressed in all comparative tissue groups, so we reason it to be suitable for all different kinds of axolotl tissue-type investigations. Moreover do we suggest GAPDH and RPLP0 as suitable for certain axolotl tissue analysis. When it comes to axolotl limb regeneration, a validated pair of reference genes is ODC and RPLP0. With these findings, new insights into axolotl gene expression profiling might be gained.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase Via Transcriptase Reversa / Genes Essenciais / Perfilação da Expressão Gênica / Ambystoma mexicanum Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: Gene Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase Via Transcriptase Reversa / Genes Essenciais / Perfilação da Expressão Gênica / Ambystoma mexicanum Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: Gene Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Holanda