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Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates.
Gharout-Sait, Alima; Touati, Abdelaziz; Guillard, Thomas; Brasme, Lucien; de Champs, Christophe.
Afiliação
  • Gharout-Sait A; Laboratoire de Microbiologie Appliquée, Département de Microbiologie, FSNV, Université de Bejaia, 06000, Algeria.
  • Touati A; Laboratoire d'Ecologie Microbienne, Département de Microbiologie, FSNV, Université de Bejaia, 06000, Algeria. Electronic address: ziz1999@yahoo.fr.
  • Guillard T; Laboratoire de Bactériologie - Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert DEBRE, Reims, France.
  • Brasme L; Laboratoire de Bactériologie - Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert DEBRE, Reims, France.
  • de Champs C; Laboratoire de Bactériologie - Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert DEBRE, Reims, France; Université de Reims-Champagne-Ardenne, Reims, France.
Braz J Infect Dis ; 19(2): 187-95, 2015.
Article em En | MEDLINE | ID: mdl-25636192
In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type ß-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates. Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4-producing isolates and one DHA-1-producing Klebsiella pneumoniae. All AmpC-producing isolates co-expressed the broad-spectrum TEM-1 ß-lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping of the E. coli isolates revealed that most of them belonged to groups D and B1. Multilocus sequence typing analysis of K. pneumoniae isolates identified four different sequence types (STs) with two new sequences: ST1617 and ST1618. Plasmid replicon typing indicates that blaCMY-4 gene was located on broad host range A/C plasmid, while LVPK replicon was associated with blaDHA-1. All isolates carrying blaCMY-4 displayed the transposon-like structures ISEcp1/ΔISEcp1-blaCMY-blc-sugE. Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates in Algeria.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cefoxitina / Enterobacteriaceae / Klebsiella pneumoniae / Antibacterianos Tipo de estudo: Screening_studies Limite: Humans País/Região como assunto: Africa Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Argélia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cefoxitina / Enterobacteriaceae / Klebsiella pneumoniae / Antibacterianos Tipo de estudo: Screening_studies Limite: Humans País/Região como assunto: Africa Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Argélia País de publicação: Brasil