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Transcriptome characterization of the ascidian Pyura chilensis using 454-pyrosequencing data from two distant localities on the southeast Pacific.
Haye, Pilar A; Gallardo-Escárate, Cristian.
Afiliação
  • Haye PA; Laboratorio de Diversidad Molecular, Departamento de Biología Marina, Facultad de Ciencias del Mar, Universidad Católica del Norte, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, Concepción, Chile; Centro de Estudios Avanzados en Zonas Áridas (CEAZA), Coquimbo, Chile. Electronic address: phaye@ucn.cl.
  • Gallardo-Escárate C; Laboratory of Biotechnology and Aquatic Genomics, University of Concepción, Concepción, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, Concepción, Chile. Electronic address: crisgallardo@udec.cl.
Mar Genomics ; 20: 19-22, 2015 Apr.
Article em En | MEDLINE | ID: mdl-25526667
This study describes the results from transcriptomes sequenced by 454-pyrosequencing from two populations separated by 10° of latitude of the endemic tunicate Pyura chilensis. Most transcripts were assembled in 43,972 contigs with an average length of 842 nucleotides. De novo assembly revealed that less than 30% of the contigs were annotated to Gene Ontology terms. A total of 71,662 single nucleotide polymorphisms (SNPs) were detected in 14,712 contigs. In silico differential expression of contigs annotated for SNPs revealed several genes differentially expressed in individuals collected from both populations. The present genomic resource will provide the basis to perform functional genomics on the species via the localization of genomic markers that can aid in determining levels of local adaptation, overall genetic structure and the genetic assessment of restocking programs for this species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Urocordados / Transcriptoma Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: Mar Genomics Ano de publicação: 2015 Tipo de documento: Article País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Urocordados / Transcriptoma Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: Mar Genomics Ano de publicação: 2015 Tipo de documento: Article País de publicação: Holanda