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Determination of cortisol and cortisone in human saliva by a liquid chromatography-tandem mass spectrometry method.
Vieira, José Gilberto Henriques; Nakamura, Odete Hirata; Carvalho, Valdemir Melechko.
Afiliação
  • Vieira JG; Steroid Laboratories, Grupo Fleury, São Paulo, SP, Brazil.
  • Nakamura OH; Steroid Laboratories, Grupo Fleury, São Paulo, SP, Brazil.
  • Carvalho VM; Steroid Laboratories, Grupo Fleury, São Paulo, SP, Brazil.
Arq Bras Endocrinol Metabol ; 58(8): 844-50, 2014 Nov.
Article em En | MEDLINE | ID: mdl-25465608
OBJECTIVE: Salivary cortisol measurement plays an important role in the evaluation of adrenal function. Its high correlation with free serum cortisol, the easy of sampling and the limited presence of interfering steroids, generated multiple recent studies of its application, in special in the screening of adrenal hyperfunction. In this paper we present our experience in the development of a high pressure liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for salivary cortisol and cortisone measurement. MATERIALS AND METHODS: For this study we used 181 saliva samples from our routine diagnostic laboratory. The HPLC-MS/MS method was based on a Waters Quattro Premier tandem mass spectrometer with an electrospray probe. After derivatization with hydroxylamine transitions monitored included cortisol and cortisone. An in-house radioimmunoassay (RIA) was used for salivary cortisol results comparison. RESULTS: Functional sensitivity was 24 ng/dL for cortisol and linearity from 24 to 1929 ng/dL. Saliva cortisol values obtained in the 181 samples presented a median of 52 ng/dL with 5-95% percentile of 24 and 374 ng/dL. With the RIA the results were 86, 25 and 436 ng/dL, respectively, with values for RIA being significantly higher (P<0.0001) and high correlation (r=0.8312, P<0.0001). Cortisone measured in 159 samples showed a median of 278 ng/dL, with 5-95% percentile of 100 and 1,133 ng/dL. Correlation with cortisol values was significant (r=0.820, P<0.0001). CONCLUSION: We conclude that the HPLC-MS/MS method compares favorably with the RIA for salivary cortisol measurement, with the additional possibility of concomitant cortisone measurement and the evaluation of 11ßHSD2 activity.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saliva / Cortisona / Hidrocortisona / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Arq Bras Endocrinol Metabol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saliva / Cortisona / Hidrocortisona / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Arq Bras Endocrinol Metabol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil