Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry.
Mem Inst Oswaldo Cruz
; 109(8): 1078-80, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25424449
Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Giardia lamblia
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Viabilidade Microbiana
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Metronidazol
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Antiprotozoários
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Portugal
País de publicação:
Brasil