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Leveraging arthropod-borne disease surveillance assays for clinical diagnostic use.
Melanson, Vanessa R; Scheirer, Jessica L; Van de Wyngaerde, Marshall T; Bourzac, Kevin; Wu, Shuenn-Jue; Kochel, Tadeusz; McAvin, James C.
Afiliação
  • Melanson VR; Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910.
  • Scheirer JL; Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910.
  • Van de Wyngaerde MT; Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910.
  • Bourzac K; BioFire Diagnostics, Inc., 39 Wakara Way, Salt Lake City, UT 84108.
  • Wu SJ; Navy Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910.
  • Kochel T; Navy Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910.
  • McAvin JC; Armed Forces Research Institute of Medical Sciences, 315/6 Rajvithi Road, Bangkok, Thailand.
Mil Med ; 179(11): 1207-11, 2014 Nov.
Article em En | MEDLINE | ID: mdl-25373042
Researchers at the Walter Reed Army Institute of Research have taken a joint service approach to filling an identified diagnostic capability gap by leveraging a vector surveillance assay. Specifically, the Army took a field-stable real-time polymerase chain reaction assay, developed by the Air Force, for dengue virus surveillance in arthropod vectors and collaborated with Navy researchers for utility in human diagnostics. As current Department of Defense diagnostic PCR assays employ the Joint Biological Agent Identification and Diagnostic System, the dengue assay was tested for use on this platform. The low rates of false negative and false positive dengue samples in clinical matrices demonstrate excellent utility as a human diagnostic assay. Overall, converting an arboviral vector surveillance assay to human diagnostic assay and potentially vice versa is both cost effective and labor reducing. Codevelopment with harmonization of vector surveillance and diagnostics offers monetary and resource advantages to the Department of Defense and should be considered as a path forward in times when downsizing threatens assay development and pathogen discovery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aedes / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Dengue / Vírus da Dengue / Insetos Vetores Tipo de estudo: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Animals / Humans País/Região como assunto: America do norte / America do sul / Peru Idioma: En Revista: Mil Med Ano de publicação: 2014 Tipo de documento: Article País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aedes / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Dengue / Vírus da Dengue / Insetos Vetores Tipo de estudo: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Animals / Humans País/Região como assunto: America do norte / America do sul / Peru Idioma: En Revista: Mil Med Ano de publicação: 2014 Tipo de documento: Article País de publicação: Reino Unido