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SASP, a Senescence-Associated Subtilisin Protease, is involved in reproductive development and determination of silique number in Arabidopsis.
Martinez, Dana E; Borniego, Maria L; Battchikova, Natalia; Aro, Eva-Mari; Tyystjärvi, Esa; Guiamét, Juan J.
Afiliação
  • Martinez DE; Instituto de Fisiología Vegetal (INFIVE) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata, Argentina danamartinez@conicet.gov.ar.
  • Borniego ML; Instituto de Fisiología Vegetal (INFIVE) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata, Argentina.
  • Battchikova N; Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
  • Aro EM; Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
  • Tyystjärvi E; Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
  • Guiamét JJ; Instituto de Fisiología Vegetal (INFIVE) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata, Argentina.
J Exp Bot ; 66(1): 161-74, 2015 Jan.
Article em En | MEDLINE | ID: mdl-25371504
Senescence involves increased expression of proteases, which may participate in nitrogen recycling or cellular signalling. 2D zymograms detected two protein species with increased proteolytic activity in senescing leaves of Arabidopsis thaliana. A proteomic analysis revealed that both protein species correspond to a subtilisin protease encoded by At3g14067, termed Senescence-Associated Subtilisin Protease (SASP). SASP mRNA levels and enzyme activity increase during leaf senescence in leaves senescing during both the vegetative or the reproductive phase of the plant life cycle, but this increase is more pronounced in reproductive plants. SASP is expressed in all above-ground organs, but not in roots. Putative AtSASP orthologues were identified in dicot and monocot crop species. A phylogenetic analysis shows AtSASP and its putative orthologues clustering in one discrete group of subtilisin proteases in which no other Arabidospsis subtilisin protease is present. Phenotypic analysis of two knockout lines for SASP showed that mutant plants develop more inflorescence branches during reproductive development. Both AtSASP and its putative rice orthologue (OsSASP) were constitutively expressed in sasp-1 to complement the mutant phenotype. At maturity, sasp-1 plants produced 25% more inflorescence branches and siliques than either the wild-type or the rescued lines. These differences were mostly due to an increased number of second and third order branches. The increased number of siliques was compensated for by a small decrease (5.0%) in seed size. SASP downregulates branching and silique production during monocarpic senescence, and its function is at least partially conserved between Arabidopsis and rice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sementes / Subtilisinas / Arabidopsis / Regulação da Expressão Gênica de Plantas / Proteínas de Arabidopsis Tipo de estudo: Risk_factors_studies Idioma: En Revista: J Exp Bot Assunto da revista: BOTANICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sementes / Subtilisinas / Arabidopsis / Regulação da Expressão Gênica de Plantas / Proteínas de Arabidopsis Tipo de estudo: Risk_factors_studies Idioma: En Revista: J Exp Bot Assunto da revista: BOTANICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido