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Mesenchymal stromal cells from adipose tissue attached to suture material enhance the closure of enterocutaneous fistulas in a rat model.
Volpe, Bruno Bosch; Santos Duarte, Adriana da Silva; Ribeiro, Thiago Borsoi; Stocchero, Ithamar; Kharmandayan, Paulo; Olalla Saad, Sara Teresinha; Bustorff-Silva, Joaquim Murray; Malheiros Luzo, Angela Cristina.
Afiliação
  • Volpe BB; Public Umbilical Cord Blood Bank, Haematology Hemotherapy Center/INCT do Sangue, University of Campinas, Campinas, Brazil.
  • Santos Duarte Ada S; Public Umbilical Cord Blood Bank, Haematology Hemotherapy Center/INCT do Sangue, University of Campinas, Campinas, Brazil.
  • Ribeiro TB; Public Umbilical Cord Blood Bank, Haematology Hemotherapy Center/INCT do Sangue, University of Campinas, Campinas, Brazil.
  • Stocchero I; Plastic Surgery Department, Faculty of Medical Sciences, University of Campinas, Campinas, Brazil.
  • Kharmandayan P; Plastic Surgery Department, Faculty of Medical Sciences, University of Campinas, Campinas, Brazil.
  • Olalla Saad ST; Internal Medicine Department, Faculty of Medical Sciences, Haematology Hemotherapy Center/INCT do Sangue, University of Campinas, Campinas, Brazil.
  • Bustorff-Silva JM; Paediatric Surgery Department, Faculty of Medical Sciences, University of Campinas, Campinas, Brazil.
  • Malheiros Luzo AC; Public Umbilical Cord Blood Bank, Haematology Hemotherapy Center/INCT do Sangue, University of Campinas, Campinas, Brazil. Electronic address: luzo@unicamp.br.
Cytotherapy ; 16(12): 1709-19, 2014 Dec.
Article em En | MEDLINE | ID: mdl-25305734
BACKGROUND AIMS: Surgical treatment for enterocutaneous fistulas (EF) frequently fails. Cell therapy may represent a new approach to treatment. Mesenchymal stromal cells (MSCs) have high proliferative and differentiation capacity. This study aimed to investigate whether MSCs could adhere to suture filament (SF), promoting better EF healing. METHODS: MSCs, 1 × 10(6), from adipose tissue (ATMSCs) were adhered to a Polyvicryl SF by adding a specific fibrin glue formulation. Adhesion was confirmed by confocal and scanning electron microscopy (SEM). A cecal fistula was created in 22 Wistar rats by incising the cecum and suturing the opening to the surgical wound subcutaneously with four separate stitches. The animals were randomly allocated to three groups: control (CG)-five animals, EF performed; injection (IG)-eight animals 1 × 10(6) ATMSCs injected around EF borders; and suture filament (SG): nine animals, sutured with 1 × 10(6) ATMSCs attached to the filaments with fibrin glue. Fistulas were photographed on the operation day and every 3 days until the 21st day and analyzed by two observers using ImageJ Software. RESULTS: Confocal and SEM results demonstrated ATMSCs adhered to SF (ATMSCs-SF). The average reduction size of the fistula area at 21st day was greater for the SG group (90.34%, P < 0.05) than the IG (71.80%) and CG (46.54%) groups. CONCLUSIONS: ATMSCs adhered to SF maintain viability and proliferative capacity. EF submitted to ATMSCs-SF procedure showed greater recovery and healing. This approach might be a new and effective tool for EF treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Suturas / Cicatrização / Tecido Adiposo / Fístula Intestinal / Proliferação de Células / Células-Tronco Mesenquimais Limite: Animals Idioma: En Revista: Cytotherapy Assunto da revista: TERAPEUTICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Suturas / Cicatrização / Tecido Adiposo / Fístula Intestinal / Proliferação de Células / Células-Tronco Mesenquimais Limite: Animals Idioma: En Revista: Cytotherapy Assunto da revista: TERAPEUTICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido