Your browser doesn't support javascript.
loading
Development, antibiotic production, and ribosome assembly in Streptomyces venezuelae are impacted by RNase J and RNase III deletion.
Jones, Stephanie E; Leong, Vivian; Ortega, Joaquin; Elliot, Marie A.
Afiliação
  • Jones SE; Department of Biology and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada.
  • Leong V; Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada.
  • Ortega J; Department of Biochemistry and Biomedical Sciences and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada.
  • Elliot MA; Department of Biology and the Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada melliot@mcmaster.ca.
J Bacteriol ; 196(24): 4253-67, 2014 Dec.
Article em En | MEDLINE | ID: mdl-25266378
RNA metabolism is a critical but frequently overlooked control element affecting virtually every cellular process in bacteria. RNA processing and degradation is mediated by a suite of ribonucleases having distinct cleavage and substrate specificity. Here, we probe the role of two ribonucleases (RNase III and RNase J) in the emerging model system Streptomyces venezuelae. We show that each enzyme makes a unique contribution to the growth and development of S. venezuelae and further affects the secondary metabolism and antibiotic production of this bacterium. We demonstrate a connection between the action of these ribonucleases and translation, with both enzymes being required for the formation of functional ribosomes. RNase III mutants in particular fail to properly process 23S rRNA, form fewer 70S ribosomes, and show reduced translational processivity. The loss of either RNase III or RNase J additionally led to the appearance of a new ribosomal species (the 100S ribosome dimer) during exponential growth and dramatically sensitized these mutants to a range of antibiotics.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleases / Ribossomos / Streptomyces / Antibacterianos País/Região como assunto: America do sul / Venezuela Idioma: En Revista: J Bacteriol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Canadá País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleases / Ribossomos / Streptomyces / Antibacterianos País/Região como assunto: America do sul / Venezuela Idioma: En Revista: J Bacteriol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Canadá País de publicação: Estados Unidos